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Reconstruction method for extended depth-of-field limited-angle tomography

机译:扩展景深限制角层析成像的重建方法

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Limited-angle optical diffraction tomography (LAODT) is a powerful tool for measuring 3D refractive index distribution in biological microsamples. However, when thick objects are measured, reconstructions are erroneous due to diffraction errors even in the case when tomographic reconstruction algorithms take into account this phenomenon. We propose a hardware-based solution which allows to change a focal plane position with a liquid tunable lens in LAODT system. For each illumination angle, projections with different focal plane positions are recorded, and thus diffraction errors in the neighborhood of these planes are minimized. In this paper, we describe a method for processing data from a varifocal tomography setup that utilizes a Generalized Total Variation Iterative Constraint algorithm.
机译:有限角光学衍射层析成像(LAODT)是一种用于测量生物微量样品中3D折射率分布的强大工具。然而,当测量厚的物体时,即使在层析重建算法考虑到这种现象的情况下,由于衍射误差,重建也是错误的。我们提出了一种基于硬件的解决方案,该解决方案允许在LAODT系统中使用液体可调透镜来更改焦平面位置。对于每个照明角度,将记录具有不同焦平面位置的投影,从而使这些平面附近的衍射误差最小。在本文中,我们描述了一种使用广义总变差迭代约束算法处理自变焦层析成像数据的方法。

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