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首页> 外文会议>Quantitative phase imaging >Coherence-controlled holographic microscopy for live-cell quantitative phase imaging
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Coherence-controlled holographic microscopy for live-cell quantitative phase imaging

机译：相干控制全息显微镜用于活细胞定量相成像

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摘要

In this paper we present coherence-controlled holographic microscopy (CCHM) and various examples of observations of living cells including combination of CCHM with fluorescence microscopy. CCHM is a novel technique of quantitative phase imaging (QPI). It is based on grating off-axis interferometer, which is fully adapted for the use of incoherent illumination. This enables high-quality QPI free from speckles and parasitic interferences and lateral resolution of classical widefield microscopes. Label-free nature of QPI makes CCHM a useful tool for long-term observations of living cells. Moreover, coherence-gating effect induced by the use of incoherent illumination enables QPI of cells even in scattering media. Combination of CCHM with common imaging techniques brings the possibility to exploit advantages of QPI while simultaneously identifying the observed structures or processes by well-established imaging methods. We used CCHM for investigation of general parameters of cell life cycles and for research of cells reactions to different treatment. Cells were also visualized in 3D collagen gel with the use of CCHM. It was found that both the cell activity and movement of the collagen fibers can be registered. The method of CCHM in combination with fluorescence microscopy was used in order to obtain complementary information about cell morphology and identify typical morphological changes associated with different types of cell death. This combination of CCHM with common imaging technique has a potential to provide new knowledge about various processes and simultaneously their confirmation by comparison with known imaging method.
机译：在本文中，我们介绍了相干控制全息显微镜（CCHM）和活细胞观察的各种示例，包括CCHM与荧光显微镜的组合。 CCHM是定量相位成像（QPI）的新技术。它基于光栅离轴干涉仪，完全适用于非相干照明。这样就可以实现高质量的QPI，而没有斑点，寄生干扰和传统宽视野显微镜的横向分辨率。 QPI的无标签性质使CCHM成为长期观察活细胞的有用工具。此外，通过使用非相干照明引起的相干门控效应即使在散射介质中也能实现细胞的QPI。 CCHM与常见成像技术的结合带来了利用QPI优势的可能性，同时可以通过完善的成像方法识别观察到的结构或过程。我们使用CCHM来研究细胞生命周期的一般参数以及研究细胞对不同处理的反应。还使用CCHM在3D胶原蛋白凝胶中可视化细胞。发现胶原纤维的细胞活性和运动都可以被记录。使用CCHM与荧光显微镜相结合的方法，以获得有关细胞形态的补充信息，并确定与不同类型的细胞死亡相关的典型形态变化。 CCHM与常见成像技术的这种结合具有提供有关各种过程的新知识的潜力，并且可以通过与已知成像方法进行比较来同时确认它们。

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  • 来源
    《Quantitative phase imaging》|2015年|93360K.1-93360K.4|共4页
  • 会议地点 San Francisco CA(US)
  • 作者

    Tomas Slaby; Aneta Krizova; Martin Lostak; Jana Collakova; Veronika Juzova; Pavel Vesely; Radim Chmelik;

  • 作者单位

    Institute of Physical Engineering, Faculty of Mechanical Engineering, Brno University of Technology, Technicka 2, 616 00 Brno, Czech Republic,TESCAN Brno, s.r.o., Libusina tr. 1, 623 00 Brno, Czech Republic;

    Institute of Physical Engineering, Faculty of Mechanical Engineering, Brno University of Technology, Technicka 2, 616 00 Brno, Czech Republic,TESCAN Brno, s.r.o., Libusina tr. 1, 623 00 Brno, Czech Republic;

    Institute of Physical Engineering, Faculty of Mechanical Engineering, Brno University of Technology, Technicka 2, 616 00 Brno, Czech Republic,TESCAN Brno, s.r.o., Libusina tr. 1, 623 00 Brno, Czech Republic;

    Institute of Physical Engineering, Faculty of Mechanical Engineering, Brno University of Technology, Technicka 2, 616 00 Brno, Czech Republic,Central European Institute of Technology, Brno University of Technology, Technicka 10, 616 00 Brno, Czech Republic;

    TESCAN Brno, s.r.o., Libusina tr. 1, 623 00 Brno, Czech Republic;

    Central European Institute of Technology, Brno University of Technology, Technicka 10, 616 00 Brno, Czech Republic;

    Institute of Physical Engineering, Faculty of Mechanical Engineering, Brno University of Technology, Technicka 2, 616 00 Brno, Czech Republic,Central European Institute of Technology, Brno University of Technology, Technicka 10, 616 00 Brno, Czech Republic;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    holographic microscope; quantitative phase imaging; interference microscope; live cell; fluorescence microscopy; digital holography; digital holographic microscopy; incoherent holography;

    机译：全息显微镜定量相成像干涉显微镜活细胞荧光显微镜数字全息术数字全息显微镜；非相干全息;

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