• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文会议>Reporters, markers, dyes, nanoparticles, and molecular probes for biomedical applications IV >Calibrating the Imaging and Therapy Performance of Magneto- fluorescent Gold Nanoshells for Breast Cancer
【24h】

Calibrating the Imaging and Therapy Performance of Magneto- fluorescent Gold Nanoshells for Breast Cancer

机译：磁荧光金纳米壳在乳腺癌中的成像和治疗性能的校准

获取原文
获取原文并翻译 | 示例
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Gold nanoshells with NIR plasmon resonance can be modified to simultaneously enhance conjugated NIR fluorescence dyes and T2 contrast of embedded iron-oxide nanoparticles, and molecularly targeted to breast and other cancers. We calibrated the theranostic performance of magneto-fluorescent nanoshells, and contrasted the performance of molecularly targeted and untargeted nanoshells for breast cancer therapy, employing MCF-7L and their HER2 over-expressing derivative MCF-7/HER2-18 breast cancer cells as in vitro model systems. Silica core gold nanoshells with plasmon resonance on ～810 nm were doped with NIR dye ICG and ~10 nm iron-oxide nanoparticles in a ～20 nm epi-layer of silica. A subset of nanoshells was conjugated to antibodies targeting HER2. Cell viability with varying laser power levels in presence and absence of bare and HER2-targeted nanoshells was assessed by calcein and propidium iodide staining. For MCF-7L cells, increasing power resulted in increased cell death (F=5.63, p=0.0018), and bare nanoshells caused more cell death than HER2-targeted nanoshells or laser treatment alone (F=30.13, p<0.001). For MCF-7/HER2-18 cells, death was greater with HER2-targeted nanoshells and was independent of laser power. This study demonstrates the capability of magneto-fluorescent nanocomplexes for imaging and therapy of breast cancer cells, and the advantages of targeting receptors unique to cancer cells.
机译：可以修饰具有NIR等离振子共振的金纳米壳，以同时增强共轭NIR荧光染料和嵌入式氧化铁纳米颗粒的T2对比，并且分子靶向乳腺癌和其他癌症。我们校准了磁荧光纳米壳的诊断性能，并对比了分子靶向和非靶向纳米壳在乳腺癌治疗中的性能，采用了MCF-7L及其HER2过表达衍生的MCF-7 / HER2-18乳腺癌细胞作为体外实验模型系统。在约〜20 nm的硅外延层中掺入了等离振子共振在〜810 nm处的二氧化硅核心金纳米壳。纳米壳的子集与靶向HER2的抗体缀合。通过钙黄绿素和碘化丙锭染色评估在存在和不存在裸露的和HER2靶向的纳米壳的情况下，在不同激光功率水平下的细胞活力。对于MCF-7L细胞，功率增加导致细胞死亡增加（F = 5.63，p = 0.0018），裸露的纳米壳比靶向HER2的纳米壳或单独进行激光处理引起的细胞死亡更多（F = 30.13，p <0.001）。对于MCF-7 / HER2-18细胞，HER2靶向的纳米壳的死亡更大，并且与激光功率无关。这项研究证明了磁荧光纳米复合物对乳腺癌细胞成像和治疗的能力，以及靶向癌细胞特有受体的优势。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号