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Biomedical applications involving multiphoton probes

机译：涉及多光子探针的生物医学应用

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摘要

Many techniques in biological and clinical science use multiphoton absorbers for fluorescence. The applications include medical imaging for living cells, diagnostic techniques for disease and spectroscopy. The intrinsic value of the multiphoton absorber coefficients is therefore of the utmost importance. Additionally, the laser intensity at which the absorber saturates can determine which absorber, dye or protein is useful for a particular application. Yet, experimental methods for determining the optical coefficients often yield different results. We describe several common methods of 2PA measurements and describe their features. As an example of the importance of applying the correct analysis to measurements, we fit experimental data and obtain values for multiphoton absorbers and accurately obtain their intrinsic values. Finally, we present the optical properties of several multiphoton materials used in biology.
机译：生物和临床科学中的许多技术都使用多光子吸收剂来产生荧光。其应用包括活细胞医学成像，疾病和光谱学诊断技术。因此，多光子吸收系数的内在价值至关重要。另外，吸收剂饱和的激光强度可以确定哪种吸收剂，染料或蛋白质可用于特定应用。但是，用于确定光学系数的实验方法通常会产生不同的结果。我们描述了2PA测量的几种常见方法并描述了它们的功能。作为对测量进行正确分析的重要性的一个示例，我们拟合了实验数据并获得了多光子吸收体的值，并准确获得了其内在值。最后，我们介绍了几种用于生物学的多光子材料的光学性质。

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