首页> 外文会议>Researches and Progresses of Modern Technology on Silk, Textile and Mechanicals >Role of IAP Gene of Bombyx mori in the Process of Baculovirus Infection
【24h】

Role of IAP Gene of Bombyx mori in the Process of Baculovirus Infection

机译:家蚕IAP基因在杆状病毒感染过程中的作用

获取原文
获取原文并翻译 | 示例

摘要

Inhibition of apoptosis protein (iap) factor was amplificated from silkworm using RT-PCR. A recombinant baculovirus, carrying iap gene of Bombyx mori was successfully constructed for the large-scale production of IAP protein by Bac-to-Bac system. DNA of the recombinant baculovirus was transfected into Bm cells of the silkworm recombinant virus. Cell morphology was observed and cell survival rate was calculated at different time post infection. The results showed that the iap gene possessed anti- apoptotic role in the process of baculovirus infection. Over-expression of the iap gene at host cells could extend the life span and life cycle of virus.
机译:使用RT-PCR从家蚕中扩增凋亡蛋白(iap)因子的抑制作用。成功构建了携带家蚕Iap基因的重组杆状病毒,通过Bac-to-Bac系统大规模生产IAP蛋白。将重组杆状病毒的DNA转染到家蚕重组病毒的Bm细胞中。在感染后的不同时间观察细胞形态并计算细胞存活率。结果表明,iap基因在杆状病毒感染过程中具有抗凋亡作用。 Iap基因在宿主细胞中的过度表达可以延长病毒的寿命和生命周期。

著录项

  • 来源
  • 会议地点 Suzhou(CN);Suzhou(CN)
  • 作者

    Shengxiang She; rnChaoqun Ma;

  • 作者单位

    Wu Hui-ling@Institute of Life Sciences, Jiangsu University, Zhenjiang 212013, China--LI Xi-bo@Institute of Life Sciences, Jiangsu University, Zhenjiang 212013, China--Li Bing@School of Life Sciences, Soochow University, Suzhou 215123, China--Shen Wei-de@School of Life Sciences, Soochow University, Suzhou 215123, China--Wang Wen-bing@Institute of Life Sciences, Jiangsu University, Zhenjiang 212013, China--;

  • 会议组织
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 纺织工业、染整工业;
  • 关键词

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱:kefu@zhangqiaokeyan.com

京公网安备:11010802029741号 ICP备案号:京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有
  • 客服微信

  • 服务号