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首页> 外文会议>Scientia pharmaceutica >Viability of the Human Adenocarcinoma Cell Line Caco-2: Influence of Cryoprotectant, Freezing Rate, and Storage Temperature
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Viability of the Human Adenocarcinoma Cell Line Caco-2: Influence of Cryoprotectant, Freezing Rate, and Storage Temperature

机译：人腺癌细胞系Caco-2的生存力：低温保护剂，冷冻速率和储存温度的影响

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摘要

The freezing process as well as cryostorage may induce molecular and cellular changes due to osmotic stress. Currently, standard protocols for cryopreservation of mammalian cells recommend slow freezing and rapid thawing to avoid intracellular ice crystal formation and osmotic damage. As an appropriate freezing process should guarantee adequate cell viability, we evaluated the impact of two cryoprotectants, a commercially available cryopreservation medium, two freezing rates, as well as two storage temperatures on the viability of Caco-2 cells. The freezing parameters were optimized by carefully determining vitality, cell count, proliferation, and functional differentiation of the cells. Though at least 90% of the cells were viable after one freezing/thawing cycle, adequate recovery of proliferation and differentiation is obtained not until 10 days post thawing. Interestingly, addition of 10% PEG 200 as cryoprotectant yielded best results. All in all, our results should give valuable advice for the optimization of the cryopreservation protocol of individual cell lines.
机译：冷冻过程以及冷冻保鲜剂可能由于渗透压而引起分子和细胞变化。当前，冷冻保存哺乳动物细胞的标准方案建议缓慢冷冻和快速融化，以避免细胞内冰晶的形成和渗透破坏。由于适当的冷冻过程应保证足够的细胞存活力，因此我们评估了两种冷冻保护剂，市售冷冻保存介质，两种冷冻速率以及两种储存温度对Caco-2细胞存活力的影响。通过仔细确定细胞的活力，细胞数，增殖和功能分化来优化冷冻参数。尽管在一个冷冻/融化循环后至少有90％的细胞能够存活，但是直到融化后10天才获得了足够的增殖和分化恢复。有趣的是，添加10％PEG 200作为防冻剂可获得最佳结果。总而言之，我们的结果应为优化单个细胞系的冷冻保存方案提供有价值的建议。

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  • 来源
    《Scientia pharmaceutica》|2009年|133-141|共9页
  • 会议地点 Vienna(AT)
  • 作者

    Iris Guell; Paula Marques Alves; Franz Gabor; Michael Wirth;

  • 作者单位

    Department of Pharmaceutical Technology and Biopharmaceutics, University of Vienna, Althanstrafie 14, 1090, Vienna, Austria;

    ITQB-UNL/IBET, Apartado 12, 27801-901, Oeiras, Portugal;

    Department of Pharmaceutical Technology and Biopharmaceutics, University of Vienna, Althanstrafie 14, 1090, Vienna, Austria;

    Department of Pharmaceutical Technology and Biopharmaceutics, University of Vienna, Althanstrafie 14, 1090, Vienna, Austria;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 药学;
  • 关键词

    Caco-2; cryopreservation; freezing rate; cryoprotectant; viability;

    机译：Caco-2；冷冻保存冻结率防冻剂;可行性;

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