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首页> 外文会议>Second International Conference on Sustainable Agriculture for Food, Energy and Industry Vol.2 >Population Fluctuation of Antagonistic Bacteria from Rice Seed Surface during Rice Growing Stages
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Population Fluctuation of Antagonistic Bacteria from Rice Seed Surface during Rice Growing Stages

机译：水稻生育期水稻种子表面拮抗细菌的种群波动

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Strains of bacterial antagonists to rice blast caused by Pyricularia grisea were isolated from the rice seed surface sampling from postharvest to storage time. The antagonistic bacteria after screening for rice blast control were identified by cell fatty acid analysis and according to the morphological, physiological and biochemical characteristics. AH of similarities were over 0.607.They were four strains belonged to Bacillus subtilis, there B. megaterium, the other three B. pumilus, one B. cereus and the other one B. licheniformis. There were a quite different population fluctuation of bacterial biomass on the surface of rice seeds during storage time, before and after seed germination, and also on the seedlings between paddy-breeding and upland-breeding. It was found that at the beginning of storage time the density of bacterial biomass on seeds at 10~8cfu/ml.g, the population of antagonists were higher at 80-100% of bacterial biomass. With the time after 3-4 months storage the number of biomass of bacteria was dramatically increased up to 10~(10) cfu/ml.g seeds while the antagonistical bacteria were decreased. To the end of storage time, 7month in total, the biomass of bacteria were still kept at 10~9 cfu/ml.g seeds, but the antagonistic bacteria were very few. For the fungi it was showed that at the middle of rice seeds storage time the fungi were started increasing, and to the end of storage time the fungus was actively but the antagonists were very few. The population of microbial biomass and the antagonistic bacteria from upland-breeding rice seedlings quite clearly higher than paddy-breeding seedlings. The population density of microorganisms on a germinated seeds was significantly higher than that before germinated, and as the same manner for antagonistic bacteria.
机译：从收获后至贮藏时间的水稻种子表面采样中分离出由稻瘟病菌引起的稻瘟病细菌拮抗剂菌株。通过细胞脂肪酸分析并根据形态，生理和生化特征，鉴定了筛选稻瘟病对照后的拮抗细菌。 AH的相似性超过0.607。它们是枯草芽孢杆菌的四个菌株，其中有巨大芽孢杆菌，另外三个短小芽孢杆菌，蜡状芽孢杆菌和另一个地衣芽孢杆菌。在贮藏期间，种子萌发前后，以及水稻育种和旱地育种之间，水稻种子表面细菌生物量的种群波动差异很大。发现在贮藏开始时，种子上细菌生物量的密度为10〜8cfu / ml.g，拮抗剂的数量较高，为细菌生物量的80-100％。随着3-4个月的储存时间，细菌的生物量急剧增加，达到10〜（10）cfu / ml.g种子，而拮抗细菌减少。到贮藏期结束为止，总共7个月，细菌的生物量仍保持在10〜9 cfu / ml.g种子，但拮抗细菌很少。对于真菌而言，表明在水稻种子的贮藏时间中，真菌开始增加，到贮藏时间结束时，真菌是活跃的，但拮抗剂很少。旱地水稻秧苗的微生物量和拮抗细菌的数量明显高于水稻秧苗。发芽种子上微生物的种群密度明显高于发芽前，并且与拮抗细菌相同。

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