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首页> 外文会议>Second International Conference on Sustainable Agriculture for Food, Energy and Industry Vol.1 >Genetic Modification with BADH Gene to Improve the Salt Tolerance of Tomato
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Genetic Modification with BADH Gene to Improve the Salt Tolerance of Tomato

机译：BADH基因的遗传修饰可提高番茄的耐盐性

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摘要

Glycinebetaine is an important compatible solute in response to salt and other osmotic stresses in many organisms. Its synthesis needs the catalysis of betaine aldehyde dehydrogenase encoded by BADH gene that converts betaine aldehyde into glycinebetaine in some halotolerant plants. BADH gene from Atriplex hortensis was introduced into tomato (Lycopersicon esculentum cv. Bailichun) via Agrobacterium-mediated transformation. PCR and Southern hybridization analysis demonstrated that the exogenous BADH gene had integrated into the genome of tomato. Transgenic tomato plants showed higher levels of mRNA and BADH activity than the wild type, but the levels are significantly different. Observations of rooting development and relative electronic conductivity examination demonstrated that the transgenic plants acquired tolerance to salt stress. Plants of two TO lines with high levels of expression of BADH gene were self-pollinated so as to obtain their T1 progenies. Test of kanamycin tolerance of T1 seedlings demonstrated that the tolerant and sensitive progenies fit a 3:1 segregation ratio. Salt tolerance evaluation of the progenies was performed in vitro and in soil-less condition. Germination ratio and growth status of the transgenic lines was improved significantly under salt stress. Transgenic seedlings showed a higher level of root production and differentiation ratio than wild type on NaCl-supplemented medium.
机译：甘氨酸甜菜碱是一种重要的相容性溶质，可应对许多生物体中的盐分和其他渗透胁迫。它的合成需要催化由BADH基因编码的甜菜碱醛脱氢酶，该酶将甜菜碱醛转化为某些卤代植物中的甘氨酸甜菜碱。通过农杆菌介导的转化，将麦冬的BADH基因引入番茄（番茄Lycopersicon esculentum cv。Bailichun）。 PCR和Southern杂交分析表明，外源BADH基因已整合到番茄基因组中。转基因番茄植株比野生型植株具有更高的mRNA和BADH活性，但水平却存在显着差异。生根发育和相关电导率检查的观察结果表明，转基因植物获得了对盐胁迫的耐受性。对两个具有高表达BADH基因的TO系的植物进行自花授粉，以获得其T1后代。卡那霉素对T1幼苗的耐受性测试表明，耐受和敏感的后代符合3：1的分离比。后代的耐盐性评估是在体外和无土条件下进行的。在盐胁迫下，转基因品系的发芽率和生长状况得到明显改善。在添加NaCl的培养基上，转基因幼苗的根系生成和分化比水平高于野生型。

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