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首页> 外文会议>Sensing for agriculture and food quality and safety V >Time Efficient Methods for Scanning a Fluorescent Membrane with a Fluorescent Microscopic Imager for the Quality Assurance of Food
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Time Efficient Methods for Scanning a Fluorescent Membrane with a Fluorescent Microscopic Imager for the Quality Assurance of Food

机译：使用荧光显微成像仪扫描荧光膜以节省食品质量的省时方法

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An important part of the quality assurance of meat is the estimation of germs in the meat exudes. The kind and the number of the germs in the meat affect the medical risk for the consumer of the meat. State-of-the-art analyses of meat are incubator test procedures. The main disadvantages of such incubator tests are the time consumption, the necessary equipment and the need of special skilled employees. These facts cause in high inspection cost. For this reason a new method for the quality assurance is necessary which combines low detection limits and less time consumption. One approach for such a new method is fluorescence microscopic imaging. The germs in the meat exude are caught in special membranes by body-antibody reactions. The germ typical signature could be enhanced with fluorescent chemical markers instead of reproduction of the germs. Each fluorescent marker connects with a free germ or run off the membrane. An image processing system is used to detect the number of fluorescent particles. Each fluorescent spot should be a marker which is connected with a germ. Caused by the small object sizes of germs, the image processing system needs a high optical magnification of the camera. However, this leads to a small field of view and a small depth of focus. For this reasons the whole area of the membrane has to be scanned in three dimensions. To minimize the time consumption, the optimal path has to be found. This optimization problem is influenced by features of the hardware and is presented in this paper. The traversing range in each direction, the step width, the velocity, the shape of the inspection volume and the field of view have influence on the optimal path to scan the membrane.
机译：肉品质保证的重要部分是对肉中细菌的估计。肉中细菌的种类和数量影响食用肉者的医疗风险。肉类的最新分析是孵化器测试程序。这种孵化器测试的主要缺点是耗时，必需的设备以及需要专门技能的员工。这些事实导致高检查成本。因此，需要一种新的质量保证方法，该方法将低检测限和更少的时间消耗结合在一起。这种新方法的一种方法是荧光显微成像。肉类中的细菌通过人体抗体反应被捕获在特殊的膜中。可以用荧光化学标记物代替细菌的繁殖来增强细菌的典型特征。每个荧光标记物都与游离细菌连接或从膜上流出。图像处理系统用于检测荧光颗粒的数量。每个荧光斑应是与细菌连接的标记。由于细菌的物体尺寸小，图像处理系统需要相机的高光学倍率。但是，这导致视野较小和焦点深度较小。因此，必须在三个维度上扫描膜的整个区域。为了减少时间消耗，必须找到最佳路径。该优化问题受硬件功能的影响，并在本文中进行了介绍。每个方向上的移动范围，步长，速度，检查体积的形状和视场都会影响扫描膜的最佳路径。

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