首页> 外文会议>Sensing for Agriculture and Food Quality and Safety VII >In-situ Detection of Multiple Pathogenic Bacteria on Food Surfaces
【24h】

In-situ Detection of Multiple Pathogenic Bacteria on Food Surfaces

机译:食品表面多种致病菌的原位检测

获取原文
获取原文并翻译 | 示例

摘要

Real-time in-situ detection of pathogenic bacteria on fresh food surfaces was accomplished with phage-based magnetoelastic (ME) biosensors. The ME biosensor is constructed of a small rectangular strip of ME material that is coated with a biomolecular recognition element (phage, antibodies or proteins, etc.) that is specific to the target pathogen. This mass-sensitive ME biosensor is wirelessly actuated into mechanical resonance by an externally applied time-varying magnetic field. When the biosensor binds with target bacteria, the mass of the sensor increases, resulting in a decrease in the sensor's resonant frequency. In order to compensate for nonspecific binding, control biosensors without phage were used in this experiment. In previous research, the biosensors were measured one by one. However, the simultaneous measurement of multiple sensors was accomplished in this research, and promises to greatly shorten the analysis time for bacterial detection. Additionally, the use of multiple biosensors enables the possibility of simultaneous detection of different pathogenic bacteria. This paper presents results of experiments in which multiple phage-based ME biosensors were simultaneously monitored. The E2 phage and JRB7 phage from a landscape phage library served as the bio-recognition element that have the capability of binding specifically with Salmonella typhimurium and B. anthracis spores, respectively. Real-time in-situ detection of Salmonella typhimurium and B. anthracis spores on food surfaces are presented.
机译:新鲜的食物表面上的病原细菌的实时原位检测是通过基于噬菌体的磁弹性(ME)生物传感器完成的。 ME生物传感器由ME材料的小矩形条构成,该条上涂有对目标病原体具有特异性的生物分子识别元件(噬菌体,抗体或蛋白质等)。这种对质量敏感的ME生物传感器通过外部施加的时变磁场无线激活为机械共振。当生物传感器与目标细菌结合时,传感器的质量会增加,从而导致传感器的共振频率降低。为了补偿非特异性结合,在该实验中使用了没有噬菌体的对照生物传感器。在以前的研究中,生物传感器是一个接一个地测量的。然而,在这项研究中完成了多个传感器的同时测量,并有望大大缩短细菌检测的分析时间。另外,使用多个生物传感器使同时检测不同的病原细菌成为可能。本文介绍了同时监测多个基于噬菌体的ME生物传感器的实验结果。来自景观噬菌体文库的E2噬菌体和JRB7噬菌体作为生物识别元件,分别具有与鼠伤寒沙门氏菌和炭疽杆菌孢子特异性结合的能力。提出了在食物表面实时检测鼠伤寒沙门氏菌和炭疽杆菌孢子的方法。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱:kefu@zhangqiaokeyan.com

京公网安备:11010802029741号 ICP备案号:京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有
  • 客服微信

  • 服务号