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Active fluid mixing with magnetic microactuators for capture of Salmonella

机译:主动流体与磁性微致动器混合以捕获沙门氏菌

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摘要

Detection of low concentrations of bacteria in food samples is a challenging process. Key to this process is the separation of the target from the food matrix. We demonstrate magnetic beads and magnetic micro-cilia based microfluidic mixing and capture, which are particularly useful for pre-concentrating the target. The first method we demonstrate makes use of magnetic microbeads held on to NiFe discs on the surface of the substrate. These beads are rotated around the magnetic discs by rotating the external magnetic field. The second method we demonstrate shows the use of cilia which extends into the fluid and is manipulated by a rotating external field. Magnetic micro-features were fabricated by evaporating NiFe alloy at room temperature, on to patterned photoresist. The high magnetic permeability of NiFe allows for maximum magnetic force on the features. The magnetic features were actuated using an external rotating magnet up to frequencies of 50Hz. We demonstrate active mixing produced by the microbeads and the cilia in a microchannel. Also, we demonstrate the capture of target species in a sample using microbeads.
机译:食品样品中低浓度细菌的检测是一个具有挑战性的过程。该过程的关键是将目标与食物基质分离。我们演示了磁珠和基于微纤毛的磁微流体混合和捕获,它们对于预浓缩目标特别有用。我们演示的第一种方法是利用磁性微珠固定在基底表面的NiFe圆盘上。通过旋转外部磁场,这些磁珠围绕磁盘旋转。我们演示的第二种方法显示了纤毛的使用,纤毛延伸到流体中并由旋转的外部场操纵。通过在室温下蒸发NiFe合金到图案化的光致抗蚀剂上来制造磁性微特征。 NiFe的高磁导率可在部件上产生最大磁力。磁性特征是使用外部旋转磁铁驱动的,频率高达50Hz。我们演示了微通道中微珠和纤毛产生的主动混合。此外,我们演示了使用微珠在样品中捕获目标物种的方法。

著录项

  • 来源
  • 会议地点 Baltimore MD(US)
  • 作者单位

    School of Mechanical Engineering, Georgia Institute of Technology, Atlanta, GA 30332;

    School of Mechanical Engineering, Georgia Institute of Technology, Atlanta, GA 30332;

    School of Mechanical Engineering, Georgia Institute of Technology, Atlanta, GA 30332;

    School of Mechanical Engineering, Georgia Institute of Technology, Atlanta, GA 30332;

    Agricultural Technology Research Program, Georgia Tech Research Institute, Atlanta, GA 30332;

    Center for Food Safety, Department of Food Science and Technology, University of Georgia, Griffin, GA 30223;

    School of Mechanical Engineering, Georgia Institute of Technology, Atlanta, GA 30332;

    School of Mechanical Engineering, Georgia Institute of Technology, Atlanta, GA 30332;

  • 会议组织
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    Magnetic beads; cilia; fluid mixing; microfluidic; preconcentration; bacteria; capture;

    机译:磁珠纤毛;流体混合;微流体预浓缩菌;捕获;

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