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In-situ Characterization of Meat Aging with Diode-Laser Raman Spectroscopy

机译：二极管激光拉曼光谱法对肉类老化的原位表征

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Due to the narrow linewidth signals and its fingerprinting nature, Raman spectra provide information about the molecular structure and composition of the samples. In this paper, the applicability of Raman spectroscopy is shown for the in-situ characterization of the aging of meat. Miniaturized diode lasers are utilized as light sources with excitation wavelengths of 671 run and 785 nm with a view to the development of a portable field device for meat. As test sample, musculus longissimus dorsi from pork was taken. The chops were stored refrigerated at 5 ℃ and Raman spectra were measured daily from slaughter up to three weeks.rnThroughout the entire period of one month, the Raman spectra preserve the basic spectral features identifying the samples as meat. More specific, the spectra exhibit gradual changes of the Raman signals and they show a time-dependent modification of the background signal which arises from a laser-induced fluorescence (LIF). To analyze the time-correlation of the complex spectra, multivariate statistical methods are employed. By means of principal components analysis (PCA) a distinction of spectra is found on the time scale between day 8 and 10. This corresponds to the transition from ripened meat to meat at and beyond the limit of inedibility. After ca. 10 days of storage at 5℃ the microbial load is overwhelming and LIF increases.rnThe results of the Raman measurements depending on the storage time of meat are discussed in the context of reference analyses which have been performed in parallel.
机译：由于窄线宽信号及其指纹特征，拉曼光谱提供了有关样品分子结构和成分的信息。在本文中，拉曼光谱的适用性被证明可用于肉类老化的原位表征。微型二极管激光器被用作激发波长为671nm和785nm的光源，以开发用于肉类的便携式现场设备。作为测试样品，从猪肉中提取了背最长肌。排骨在5℃下冷藏保存，每天从屠宰开始到三周都测量拉曼光谱。在整个一个月的时间里，拉曼光谱保留了基本的光谱特征，可将样品识别为肉。更具体地，光谱显示拉曼信号的逐渐变化，并且它们显示背景信号的随时间变化，该背景信号是由激光诱导的荧光（LIF）引起的。为了分析复杂光谱的时间相关性，采用了多元统计方法。通过主成分分析（PCA），可以在第8天到第10天之间的时间范围内发现光谱差异。这对应于从成熟肉到肉的转变，达到或超过了不可食用的极限。后约。在5℃下储存10天，微生物负荷过大，LIF增加。在平行进行的参考分析的背景下，讨论了根据肉类储存时间进行的拉曼测量结果。

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