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Analysis of Dust samples from the Middle East Using High Density Resequencing Microarray 'RPM-TEI'

机译:使用高密度重测序微阵列'RPM-TEI'分析来自中东的粉尘样品

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A previously developed resequencing microarray, "Tropical and Emerging Infections (RPM-TEI v.1.0 chip)", designed to identify and discriminate between tropical diseases and other potential biothreat agents, their near-neighbor species, and/or potential confounders, was used to characterize the microbes present in the silt/clay fraction of surface soils and airborne dust collected from the Middle East. Local populations and U.S. military personnel deployed to the Middle East are regularly subjected to high levels of airborne desert dust containing a significant fraction of inhalable particles and some portion require clinical aid. Not all of the clinical symptoms can be directly attributed to the physical action of material in the human respiratory tract. To better understand the potential health effects of the airborne dust, the composition of the microbial communities associated with surface soil and/or airborne dust (air filter) samples from 19 different sites in Iraq and Kuwait was identified using RPM-TEI v.1.0. Results indicated that several microorganisms including a class of rapidly growing Mycobacterium, Bacillus, Brucella, Clostridium and Coxiella burnetti, were present in the samples. The presence of these organisms in the surface soils and the inhalable fraction of airborne dust analyzed may pose a human health risk and warrants further investigation. Better understanding of the factors influencing the composition of these microbial communities is important to address questions related to human health and is critical to achieving Force Health Protection for the Warfighter operating in the Middle East, Afghanistan, North Africa and other arid regions.
机译:使用先前开发的重测序微阵列“热带和新兴感染(RPM-TEI v.1.0芯片)”,该芯片旨在识别和区分热带病和其他潜在的生物威胁因子,它们的近邻物种和/或潜在的混杂因素。以表征存在于从中东收集的表层土壤的粉尘/粘土部分和空气传播的粉尘中的微生物。派往中东的当地居民和美国军事人员经常要接受大量的空气传播的沙漠尘埃,其中含有可吸入颗粒物的很大一部分,而有些则需要临床援助。并非所有的临床症状都可以直接归因于人类呼吸道物质的物理作用。为了更好地了解空气传播尘埃对健康的潜在影响,使用RPM-TEI v.1.0确定了与伊拉克和科威特19个不同地点的地表土壤和/或空气传播尘埃(空气过滤器)样本相关的微生物群落组成。结果表明样品中存在几种微生物,包括一类快速生长的分枝杆菌,芽孢杆菌,布鲁氏菌,梭状芽胞杆菌和柯氏杆菌。这些有机物在表层土壤中的存在以及所分析的空气传播粉尘的可吸入部分可能构成人类健康风险,因此有必要进行进一步调查。更好地了解影响这些微生物群落组成的因素对于解决与人类健康有关的问题很重要,对于实现在中东,阿富汗,北非和其他干旱地区作战的战士的部队健康保护至关重要。

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