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Development of a microfluidic system for measuring HIV-1 viral load

机译:开发用于测量HIV-1病毒载量的微流控系统

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摘要

The World Health Organization (WHO) is rapidly expanding antiretroviral treatment (ART) in sub-Saharan countries. However, virological failure of ART is rarely monitored due to the lack of affordable and sustainable viral load assays suitable for resource-limited settings. Here, we report a prototype of a rapid virus detection method based on microfluidic technologies. In this method, HIV.1 particles from 10 μL whole blood were captured by anti-gpl20 antibody coated on the microchannel surface and detected by dual fluorescence signals under microscopy. Next, captured HIV-1 particles were counted using the free software, ImageJ. This rapid HIV-1 detection method has potential to be further developed for viral load monitoring at resource-limited settings.
机译:世界卫生组织(世卫组织)正在撒哈拉以南国家迅速扩大抗逆转录病毒治疗(ART)。然而,由于缺乏适用于资源有限环境的可负担且可持续的病毒载量测定法,很少监测ART的病毒学失败。在这里,我们报告基于微流技术的快速病毒检测方法的原型。在这种方法中,通过包被在微通道表面上的抗gp120抗体捕获来自10μL全血的HIV.1颗粒,并在显微镜下通过双重荧光信号进行检测。接下来,使用免费软件ImageJ对捕获的HIV-1颗粒进行计数。这种快速的HIV-1检测方法有潜力进一步发展,以在资源有限的环境下监测病毒载量。

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