• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文会议>Sensors, and command, control, communications, and intelligence (C3I) technologies for homeland security and homeland defense IX >The Toolbox of Fluorescence Standards: Flexible Calibration Tools for the Standardization of Fluorescence-based Measurements
【24h】

The Toolbox of Fluorescence Standards: Flexible Calibration Tools for the Standardization of Fluorescence-based Measurements

机译：荧光标准的工具箱：灵活的校准工具，用于基于荧光的测量的标准化

获取原文
获取原文并翻译 | 示例
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

To improve the reliability of fluorescence data in the life and material sciences and to enable accreditation of fluorescence techniques, standardization concepts are required that guarantee and improve the comparability of fluorescence measurements. At the core of such concepts are simple and evaluated fluorescence standards for the consideration of instrument-specific spectral and intensity distortions of measured signals and for instrument performance validation (IPV). Similarly in need are fluorescence intensity standards for the quantification from measured intensities and for signal referencing, thereby accounting for excitation light-induced intensity fluctuations. These standards should be preferably certified, especially for use in regulated areas like medical diagnostics.rnThis encouraged us to develop liquid and solid standards for different fluorescence parameters and techniques for use under routine measurement conditions in different formates. Special emphasis was dedicated to the determination and control of the spectral responsivity of detection systems, wavelength accuracy, homogeneity of illumination, and intensity referencing for e.g. spectrofluorometers, fluorescence sensors and confocal laser scanning fluorescence microscopes. Here, we will present design concepts and examples for mono- and multifunctional fluorescence standards that provide traceability to radiometric units and present a first step towards a toolbox of standards.
机译：为了提高生命科学和材料科学中荧光数据的可靠性并实现对荧光技术的认可，需要使用标准化的概念来保​​证和改善荧光测量的可比性。这些概念的核心是简单且经过评估的荧光标准，用于考虑特定于仪器的测量信号的光谱和强度失真以及仪器性能验证（IPV）。同样需要荧光强度标准，用于从测得的强度进行定量和信号参考，从而考虑激发光引起的强度波动。这些标准应优先获得认证，尤其是在诸如医学诊断之类的管制区域中使用。这鼓励我们为不同的荧光参数和技术开发液体和固体标准，以便在常规测量条件下以不同格式使用。特别着重于确定和控制检测系统的光谱响应，波长精度，照明的均匀性和强度参考，例如。分光荧光计，荧光传感器和共聚焦激光扫描荧光显微镜。在这里，我们将介绍单功能和多功能荧光标准品的设计概念和示例，这些标准品和标准品可提供辐射单位的可追溯性，并朝着标准工具箱迈出了第一步。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号