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Towards the Design of an Acetone Breath Biosensor

机译：丙酮呼吸生物传感器的设计

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摘要

Acetone is one of the most abundant volatile organic compounds (VOC) in human breath and high acetone levels result in ketoacidosis. Many acetone sensors have been developed; however, many disadvantages are present; therefore, there is a need for a more specific, biologically friendly, and simpler biosensor for breath acetone detection for diabetics. This study outlines a procedure for isolation, purification, and characterization of NADPH-dependant carbonyl reductase enzyme for use in an amperometric acetone sensor. Carbonyl reductase was extracted from Saccharomyces pastorianus yeast cells and purified using column chromatography. Carbonyl reductase activity was evaluated using activity assays and was also studied electrochemically. Furthermore, this study outlines a procedure for a flow injection method used for detecting aqueous acetone in the presence of carbonyl reductase. Lastly, various immobilization techniques were tested in order to improve current response and the selectivity towards acetone.
机译：丙酮是人类呼吸中最丰富的挥发性有机化合物（VOC）之一，丙酮含量高会导致酮症酸中毒。已经开发了许多丙酮传感器。但是，存在许多缺点。因此，需要用于糖尿病患者的呼气丙酮检测的更特异性，生物学上友好且更简单的生物传感器。这项研究概述了用于安培丙酮传感器的NADPH依赖性羰基还原酶的分离，纯化和表征方法。从巴斯德酵母酵母细胞中提取羰基还原酶，并使用柱色谱法纯化。使用活性测定法评估羰基还原酶的活性，并进行电化学研究。此外，该研究概述了用于在羰基还原酶存在下检测丙酮水溶液的流动注射方法的程序。最后，测试了各种固定技术，以改善电流响应和对丙酮的选择性。

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  • 来源
  • 会议地点 Seattle WA(US)
  • 作者

    N. Z. Hausmann; M. T. Meredith; S. D. Minteer;

  • 作者单位

    Department of Chemistry, Saint Louis University, Saint Louis, Missouri 63123, USA;

    Department of Chemistry, University of Utah, Salt Lake City, Utah 84112, USA;

    Department of Chemistry, University of Utah, Salt Lake City, Utah 84112, USA;

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  • 正文语种 eng
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