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Comparison of Background Corrected Fluorescence Correlation Spectroscopy and Fluorescence Lifetime Correlation Spectroscopy: Dilution Series Revisited

机译：背景校正的荧光相关光谱法和荧光寿命相关光谱法的比较：复数稀释系列

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We performed quantitative tests in order to compare the practical limits of FCS and FLCS. Unlike conventional FCS, FLCS yields precise and correct concentration values from as low as picomolar to micromolar concentrations. We discuss some of the inherent technical limitations of FCS and demonstrate that they are easily overcome by FLCS employing the simplest confocal detection scheme.

机译：为了比较FCS和FLCS的实际限制，我们进行了定量测试。与传统的FCS不同，FLCS可以产生精确而正确的浓度值，从低至微摩尔浓度到微摩尔浓度。我们讨论了FCS的一些固有技术局限性，并证明通过采用最简单的共聚焦检测方案，FLCS可以轻松克服它们。

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《Single molecule spectroscopy and imaging II》|2009年|718508.1-718508.6|共6页
会议地点 San Jose CA(US)
作者
Steffen Ruettinger; Peter Kapusta; Matthias Patting; Michael Wahlt; Rainer Macdonald;
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Physikalisch-Technische Bundesanstalt Abbestr 2-12 10587 Berlin;

PicoQuant GmbH Rudower Chaussee 29 12489 Berlin;

PicoQuant GmbH Rudower Chaussee 29 12489 Berlin;

PicoQuant GmbH Rudower Chaussee 29 12489 Berlin;

Physikalisch-Technische Bundesanstalt Abbestr 2-12 10587 Berlin;

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1. Dynamic assembly properties of nonmuscle myosin II isoforms revealed by combination of fluorescence correlation spectroscopy and fluorescence cross-correlation spectroscopy. [J] . Mitsuhashi M, Sakata H, Kinjo M, The Journal of Biochemistry . 2011,第3期

机译：荧光相关光谱和荧光互相关光谱的组合揭示了非肌肉肌球蛋白II亚型的动态组装特性。
2. Dynamic assembly properties of nonmuscle myosin II isoforms revealed by combination of fluorescence correlation spectroscopy and fluorescence cross-correlation spectroscopy [J] . Mariko Mitsuhashi, Hiroshi Sakata, Masataka Kinjo, Journal of Biochemistry, The . 2011,第3期

机译：荧光相关光谱和荧光互相关光谱相结合揭示了非肌肉肌球蛋白Ⅱ同工型的动态组装特性
3. Fluorescence correlation spectroscopy and fluorescence cross-correlation spectroscopy reveal the cytoplasmic origination of loaded nuclear RISC in vivo in human cells [J] . Ohrt T, Mutze J, Staroske W, Nucleic Acids Research . 2008,第20期

机译：荧光相关光谱和荧光互相关光谱揭示了人类细胞中体内核RISC的细胞质起源
4. Comparison of Background Corrected Fluorescence Correlation Spectroscopy and Fluorescence Lifetime Correlation Spectroscopy: Dilution Series Revisited [C] . Steffen Riittinger, Peter Kapusta, Matthias Patting, Conference on single molecule spectroscopy and imaging . 2009

机译：背景校正荧光相关光谱和荧光寿命相关光谱的比较：稀释系列重新审议
5. Fluorescence correlation spectroscopy and photon correlation spectroscopy of tryptophan-containing proteins in sugar solutions. [D] . Wang, Yuli. 2012

机译：糖溶液中含色氨酸的蛋白质的荧光相关光谱和光子相关光谱。
6. Fluorescence correlation spectroscopy and fluorescence cross-correlation spectroscopy reveal the cytoplasmic origination of loaded nuclear RISC in vivo in human cells [O] . Thomas Ohrt, Jörg Mütze, Wolfgang Staroske, 2008

机译：荧光相关光谱和荧光互相关光谱揭示了人类细胞中体内核RISC的细胞质起源
7. Quantitative deconvolution of autocorrelations and cross correlations from two-dimensional lifetime decay maps in fluorescence lifetime correlation spectroscopy [O] . Shanshan Gao, Menghua Cui, Ruiru Li, 2017

机译：荧光寿命相关光谱中的自相关的自相关和跨相关性的自相关和交叉相关性

Comparison of Background Corrected Fluorescence Correlation Spectroscopy and Fluorescence Lifetime Correlation Spectroscopy: Dilution Series Revisited

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