Single-molecule fluorescence inside solid-state nanochannels

机译：固态纳米通道内的单分子荧光

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One major limitation of single-molecule fluorescence spectroscopy is the finite residence time of diffusing molecules in the confocal detection volume. The time ranges in the order of milliseconds. This has typical size of femtoliter. Here, we present a concept of extending the residence time using nanochannels of ca. 60 nm × 60 nm cross-section to restrict the molecular motion. We use solid-state nanochannels of silicon and silicon dioxide. This work is aimed to use for dual-focus fluorescence detection combining Anti-Brownian ELectrophoretic or ABEL trap to actively trap single molecule.

机译：单分子荧光光谱法的一个主要限制是扩散分子在共聚焦检测体积中的有限停留时间。时间范围以毫秒为单位。这具有飞升的典型大小。在这里，我们提出了使用ca的纳米通道延长停留时间的概念。 60 nm×60 nm的横截面限制了分子运动。我们使用硅和二氧化硅的固态纳米通道。这项工作旨在用于结合防布朗电泳或ABEL陷阱来主动捕获单个分子的双焦点荧光检测。

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来源
《Single molecule spectroscopy and superresolution imaging VII》|2014年|895008.1-895008.7|共7页
会议地点 San Francisco CA(US)
作者
Siddharth Ghosh; Manoj Kumbhakar; Mitja Platen; Ingo Gregor; Joerg Enderlein;
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作者单位

International Max Planck Research School for Physics of Biological and Complex Systems, Goettingen, Germany,Ⅲrd Institute of Physics, George-August-Universitaet Goettingen, Goettinngen, Germany;

RPC Division, Molecular Laboratory, Bhabha Atomic Research Centre, Mumbai, India;

International Max Planck Research School for Physics of Biological and Complex Systems, Goettingen, Germany,Ⅲrd Institute of Physics, George-August-Universitaet Goettingen, Goettinngen, Germany;

Ⅲrd Institute of Physics, George-August-Universitaet Goettingen, Goettinngen, Germany;

Ⅲrd Institute of Physics, George-August-Universitaet Goettingen, Goettinngen, Germany;

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正文语种 eng
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关键词
Single-molecule fluorescence; nanochannel; diffusion and ABEL trap;

机译：单分子荧光；纳米通道扩散和ABEL陷阱;

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1. Inside Cover: Reversible Dimerization of EGFR Revealed by Single-Molecule Fluorescence Imaging Using Quantum Dots (Chem. Eur. J. 4/2010) [J] . Nagako Kawashima, Kenichi Nakayama Dr., Kohji Itoh Dr., Chemistry - A European Journal . 2010,第4期

机译：内部封面：使用量子点通过单分子荧光成像揭示的EGFR可逆二聚化（Chem。Eur。J. 4/2010）
2. Single-Molecule DNA Extension in Rectangular and Square Profile Nanochannels in the Extended de Gennes Regime [J] . Schotzinger R. Michael, Menard Laurent D., Ramsey J. Michael Macromolecules . 2020,第6期

机译：延长的De Gennes制度中矩形和方形剖面纳米中的单分子DNA延伸
3. Single-Molecule Detection of DNA in a Nanochannel by High-Field Strength-Assisted Electrical Impedance Spectroscopy [J] . Porpin Pungetmongkol, Takatoki Yamamoto Micromachines . 2019,第3期

机译：高电场强度辅助电阻抗谱法在纳米通道中单分子检测DNA。
4. Improved timing and diffusivity measurement in single-molecule recycling in a nanochannel [C] . Bo Wang, Lloyd M. Davis Single molecule spectroscopy and superresolution imaging X . 2017

机译：纳米通道中单分子回收中改进的时间和扩散率测量
5. Enhancing the temporal and spatial resolution of solid-state nanopore single-molecule sensors. [D] . Anderson, Brett Nels. 2013

机译：增强固态纳米孔单分子传感器的时间和空间分辨率。
6. OMSV enables accurate and comprehensive identification of large structural variations from nanochannel-based single-molecule optical maps [O] . Le Li, Alden King-Yung Leung, Tsz-Piu Kwok, 2017

机译：OMSV可以从基于纳米通道的单分子光学图上准确全面地识别大型结构变异
7. Inside Cover: Reversible Dimerization of EGFR Revealed by Single-Molecule Fluorescence Imaging Using Quantum Dots (Chem. Eur. J. 4/2010) [O] . Nagako Kawashima, Kenichi Nakayama, Kohji Itoh, 2010

机译：内覆盖：使用量子点（Chem.EUR.J. 4/2010），单分子荧光成像揭示EGFR的可逆二聚化。

Single-molecule fluorescence inside solid-state nanochannels

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