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Two methods for tracking small animals in SPECT imaging

机译:在SPECT成像中追踪小动物的两种方法

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摘要

High-resolution single photon emission computed tomography (SPECT) and X-ray computed tomography (CT) imaging have proven to be useful techniques for non-invasively monitoring mutations and disease progression in small animals. A need to perform in vivo studies of non-anesthetized animals has led to the development of a small-animal imaging system that integrates SPECT imaging equipment with a pose-tracking system. The pose of the animal is monitored and recorded during the SPECT scan using either laser-generated surfaces or infrared-reflective markers affixed to the animal. The reflective marker method measures motion by stereoscopically imaging an arrangement of illuminated markers. The laser-based method is proposed as a possible alternative to the reflector method with the advantage that it is a non-contact system. A three-step technique is described for calibrating the surface acquisition system so that quantitative surface measurements can be obtained. The acquired surfaces can then be registered to a reference surface using the iterative closest point (ICP) algorithm to determine the relative pose of the live animal and correct for any movement during the scan. High accuracy measurement results have been obtained from both methods.
机译:高分辨率单光子发射计算机断层扫描(SPECT)和X射线计算机断层扫描(CT)成像已被证明是无创监测小型动物突变和疾病进展的有用技术。对非麻醉动物进行体内研究的需求导致了一种小型动物成像系统的开发,该系统将SPECT成像设备与姿势跟踪系统集成在一起。在SPECT扫描过程中,使用激光生成的表面或贴在动物身上的红外反射标记来监视和记录动物的姿势。反射标记方法通过对照明标记的排列进行立体成像来测量运动。提出了基于激光的方法作为反射器方法的一种可能的替代方案,其优点是它是非接触式系统。描述了一种用于校准表面采集系统的三步技术,以便可以获得定量的表面测量值。然后,可以使用迭代最近点(ICP)算法将获取的表面配准到参考表面,以确定活体动物的相对姿势并校正扫描过程中的任何运动。从这两种方法都获得了高精度的测量结果。

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