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首页> 外文会议>Sixth International in Situ and On-Site Bioremediation Symposium, 6th, Jun 4-7, 2001, San Diego, California >NATURAL ATTENUATION OF NITRATE IN THE BIG DITCH WATERSHED, ILLINOIS
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NATURAL ATTENUATION OF NITRATE IN THE BIG DITCH WATERSHED, ILLINOIS

机译：伊利诺伊州大沟流域硝酸盐的自然衰减

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摘要

The focus of this research was to determine the natural attenuation capacity for nitrate in the groundwater throughout the Big Ditch Watershed. Eleven sites were sampled within the watershed and sediment samples were taken from several depths at each site. The natural attenuation capacity of the watershed was determined by enumeration of denitrifiers by most probable number (MPN) technique and by determining the intrinsic denitrification rate using a modified denitrifying enzyme activity (DEA) method. Enumeration results showed that the microorganisms were less abundant in sediment samples taken from the middle depths of the saturated zone. Three separate total carbon amendments of 2 mM, 8 mM and 20 mM were used in the denitrification rate determination. The low carbon (2mM) amendment resulted in a significantly lower denitrification rate (29.84 +- 0.25 mg N/kg soil-day) than the other carbon amendments (8mM: 132.63 +- 1.17 mg N/kg soil-day, 20mM: 193.98 +- 3.11 mg N/kg soil-day) and varied little with depth. In contrast, the denitrification rate increased with the depth of the sample for the 8 mM C and 20 mM C (193.98 +-3.11 mg N/kg soil-day) amendments. The data shows that denitrifier abundance and activity in the groundwater are dependent on the spatial variables of depth and location, the dissolved oxygen, and on the availability of carbon.
机译：这项研究的重点是确定整个大沟流域地下水中硝酸盐的自然衰减能力。在流域内对11个地点进行了采样，并从每个地点的多个深度采集了沉积物样本。分水岭的自然衰减能力是通过使用最可能数（MPN）技术枚举反硝化剂并使用改良的反硝化酶活性（DEA）方法确定内在反硝化率来确定的。枚举结果表明，从饱和带中深度采集的沉积物样品中微生物含量较低。在反硝化速率测定中使用了三个分别为2 mM，8 mM和20 mM的总碳修正量。低碳（2mM）修正导致反硝化率（29.84 +-0.25 mg N / kg土壤-天）显着低于其他碳修正（8mM：132.63 +-1.17 mg N / kg土壤-天，20mM：193.98 +-3.11 mg N / kg土壤-天），并且随深度变化不大。相反，对于8 mM C和20 mM C（193.98 + -3.11 mg N / kg土壤-天）修正剂，反硝化速率随样品深度的增加而增加。数据表明，地下水中反硝化剂的丰度和活性取决于深度和位置，溶解氧的空间变量以及碳的有效性。

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