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首页> 外文会议>Sixth International in Situ and On-Site Bioremediation Symposium, 6th, Jun 4-7, 2001, San Diego, California >BIOCATALYTIC OXIDATION OF ORGANOPHOSPHOROUS PESTICIDES BY WHITE ROT FUNGI
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BIOCATALYTIC OXIDATION OF ORGANOPHOSPHOROUS PESTICIDES BY WHITE ROT FUNGI

机译：白腐真菌对有机磷农药的生物催化氧化

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The enzymatic mechanism for the degradation of organophosphorus (OPs) pesticides by different white-rot fungi strains was studied. A collection of 17 different fungi metabolized 10% to 97% of added parathion in 96 hrs. Three strains showing the highest activities were selected for the degradation of other OPs pesticides: Bjerkandera adusta 8258, Pleurotus ostreatus 7989 and Phanerocheate chrysosporium 3641. All strains degraded terbufos, azinphos-methyl, phosmet and DEF, from 50% to 96% of these compounds in 96 hrs. In order to identify the origin of the degradation activity, extracellular fungal extracts induced for ligninolytic enzymes were evaluated. While the activity of lignin peroxidase, manganese peroxidase, and laccase were detected in the extracts, no enzymatic modification of five OPs pesticides tested could be found suggesting the intracellular origin of the degradation activity. Microsomal preparations from B. adusta 8258 were obtained and tested, the degregadation rates for the microsomal fraction were: 10 umol mg-prot~(-1) h~(-1) for phosmet, 5.7μmol mg-prot~(-1) h~(-1) for terbufos, and 2.2μmol mg-prot~(-1) h~(-1) for azinphos-methyl. These results indicate that in ligninolytic fungi the OP pesticides degradation activity is intracellular. The stringent requirement for NADPH during the degradation suggest cytochrome P450 might be involved.
机译：研究了不同的白腐真菌菌株降解有机磷农药的酶促机理。收集的17种不同真菌在96小时内代谢了10％至97％的对硫磷。选择了表现出最高活性的三种菌株来降解其他OP农药：Bjerkandera adusta 8258，Pleurotus ostreatus 7989和Phanerocheate chrysosporium3641。所有菌株均能降解萜品磷，谷硫磷，甲基磷酰胺和DEF，从这些化合物的50％到96％在96小时内。为了鉴定降解活性的起源，评估了诱导木质素分解酶的细胞外真菌提取物。虽然在提取物中检测到木质素过氧化物酶，锰过氧化物酶和漆酶的活性，但未发现对五种被测试的OPs农药进行酶促修饰，表明降解活性的细胞内来源。获得和测试了来自B. adusta 8258的微粒体制剂，微粒体级分的去调节率是：磷酸10 umol mg-prot〜（-1）h〜（-1），5.7μmolmg-prot〜（-1）对于terbufos为h〜（-1），对于谷硫磷甲基为2.2μmolmg-prot〜（-1）h〜（-1）。这些结果表明，在木质素分解真菌中，OP农药的降解活性是细胞内的。降解期间对NADPH的严格要求表明可能涉及细胞色素P450。

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