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首页> 外文会议>Sixth International in Situ and On-Site Bioremediation Symposium, 6th, Jun 4-7, 2001, San Diego, California >BIOREMEDIATION OF CREOSOTE-CONTAMINATED SOIL BY INDIGENOUS SOIL FUNGI
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BIOREMEDIATION OF CREOSOTE-CONTAMINATED SOIL BY INDIGENOUS SOIL FUNGI

机译：原生土壤真菌对菜籽油污染土壤的生物修复

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摘要

Fungal isolates from a creosote contaminated land and from the soil of an uncontaminated forest floor were tested for their capabilities to colonize and degrade creosote in a soil contaminated with >25,000 mg kg~(-1) (w/w) creosote. The fungi included two strains of basidiomycete both of which were Pleurotus sp., one from the contaminated soil and the other from an uncontaminated forest floor, and four strains of non-basidiomycete fungi, Cladosporium sp., Fusarium sp., Penicillium sp. and Aspergillus sp., from the contaminated soil. Soil analyses was done by Soxhlet extraction and gas chromatography (Eriksson et al,2000), to effect the separation of volatile and heavy components of creosote. The non-basidiomycete were found to remove between 78% and 88% creosote from the soil within the 70 days of treatment. Pleurotus sp. was found to remove up to 94% of the initial creosote concentration. A composite mixture of all the isolates from the contaminated soil showed better creosote removal from the soil. Barley as a bulking agent, N and P supplementation and aeration were observed to influence the rate of growth of all the fungi and improve on the removal of creosote from the soil. The results showed that fungi can be effectively used to remediate creosote contaminated soils provided that adequate conditions are provided. Studies are presently underway to find means of sustaining the growth of the fungi for longer periods for use in large scale application in bioremediation.
机译：测试了来自受杂酚油污染的土地和未被污染的森林地面土壤的真菌分离物在受> 25,000 mg kg〜（-1）（w / w）杂酚油污染的土壤中定殖和降解杂酚油的能力。该真菌包括两种担子菌属菌株，均是Pleurotus sp。，一种来自受污染的土壤，另一种来自未受污染的森林地面，以及四株非担子菌属真菌，Cladosporium sp。，Fusarium sp。，Penicillium sp.。和Aspergillus sp。，来自受污染的土壤。土壤分析通过索氏提取和气相色谱法进行（Eriksson等，2000），以实现杂酚油中挥发性成分和重质成分的分离。发现在处理的70天内，非担子菌可以从土壤中去除78％至88％的杂酚油。平菇被发现去除了最初的杂酚油浓度的94％。来自受污染土壤的所有分离物的复合混合物显示出从土壤中更好的杂酚油去除。大麦作为填充剂，氮和磷的补充和通气会影响所有真菌的生长速度，并改善土壤中杂酚的去除。结果表明，只要提供适当的条件，真菌可以有效地用于治理被杂酚油污染的土壤。目前正在进行研究以寻找在更长时期内维持真菌生长的方法，以用于生物修复的大规模应用。

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