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首页> 外文会议>Smart Structures, Devices, and Systems III; Proceedings of SPIE-The International Society for Optical Engineering; vol.6769 >Supported lipid bilayers membranes on SiO_2 and TiO_2: Substrate effects on membrane formation and shape transformation
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Supported lipid bilayers membranes on SiO_2 and TiO_2: Substrate effects on membrane formation and shape transformation

机译：SiO_2和TiO_2上支持的脂质双层膜：基质对膜形成和形状转变的影响

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摘要

Lipid bilayer membranes deposited on solid surfaces are called "supported planar bilayers" (SPBs), and expected to be an effective cell-membrane-mimicking model system in vitro. We have investigated the influence of the substrate surface properties on the SPB formation process and on the photo-induced shape transformation of bilayers, by means of atomic force microscopy and fluorescence microscopy. The SPB of dipalmitoleoylphosphatidylcholine was formed on SiO_2/Si(100) surfaces and rutile-TiO_2(100) surface by the vesicle fusion method. On the SiO_2 surface, one or a few adsorbed vesicles can transform to a SPB resulting in a small bilayer patches. The SPB formation rate was accelerated on thermally treated SiO_2 surfaces, which had less hydrophilicity, but the initial SPB formation process did not change. On the TiO_2(100), the surface was completely covered with the adsorbed vesicles prior to the SPB formation, and the planar bilayer was obtained only if the lipid concentration in the suspension was sufficiently high. Photo-induced activation of molecular motion through the fluorescence dye excitation achieved the area-selective SPB formation from the adsorbed vesicular layers with small SPB domains on the TiO_2(100). This photo-activation transformed the SPB shapes three-dimensionally on the SiO_2 and TiO_2 surfaces in different way.
机译：沉积在固体表面上的脂质双层膜称为“支撑平面双层”（SPB），有望在体外成为有效的细胞膜模拟模型系统。我们已经通过原子力显微镜和荧光显微镜研究了基材表面性能对SPB形成过程和双层的光致形状转变的影响。通过囊泡融合法在SiO_2 / Si（100）表面和金红石-TiO_2（100）表面上形成了二棕榈油酰磷脂酰胆碱的SPB。在SiO_2表面上，一个或几个吸附的囊泡可以转变为SPB，从而形成小的双层膜片。在亲水性较低的热处理SiO_2表面上，SPB的形成速度加快，但是初始SPB的形成过程没有改变。在TiO_2（100）上，在SPB形成之前，表面完全被吸附的囊泡覆盖，只有悬浮液中的脂质浓度足够高时，才能获得平面双层。通过荧光染料激发光诱导的分子运动的激活实现了由TiO_2（100）上具有小的SPB域的吸附水泡层形成的区域选择性SPB形成。这种光活化以不同的方式在SiO_2和TiO_2表面上三维地改变了SPB形状。

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