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首页> 外文会议>Society for Biomaterials Transaction Annual Meeting vol.29 pt.2 >Evaluation of Microcarrier Formulations for Propagation of Osteoblasts and Chondrocytes
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Evaluation of Microcarrier Formulations for Propagation of Osteoblasts and Chondrocytes

机译：用于成骨细胞和软骨细胞繁殖的微载体制剂的评价

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Retrieval of osteoblasts and chondrocytes from bone and cartilage became possible with availability of enzymes to break down the extracellular matrix without lethal damage to cells. Since a limited number of cells are obtained from the enzymatic treatment of tissue, techniques to promote cell multiplication have been developed to maximize retrieval success. One of the most common techniques to propagate cells is by monolayer culture. Although cells multiply, they undergo phenotypic changes. In particular, chondrocytes assume a fibroblast-like morphology. They shift from producing type Ⅱ to type Ⅰ collagen, and they decrease their synthesis of high molecular weight proteoglycans in monolayer culture. A strategy to. promote chondrocyte proliferation while maintaining the original phenotype was developed by using a microcarrier spinner culture system. The present study tested the hypothesis that collagen-based microcarriers are the most suitable formulation for the propagation of osteoblasts and chondrocytes.
机译：随着酶的存在，可以分解骨细胞和软骨细胞，从而破坏细胞外基质，而不会对细胞造成致命的伤害。由于从组织的酶处理中获得的细胞数量有限，因此已开发出促进细胞增殖的技术以最大程度地提高检索成功率。繁殖细胞的最常见技术之一是单层培养。尽管细胞繁殖，但它们会发生表型变化。特别地，软骨细胞呈现成纤维细胞样形态。它们从产生的Ⅱ型胶原转变为Ⅰ型胶原，并且在单层培养中减少了高分子量蛋白聚糖的合成。一个策略。通过使用微载体旋转培养系统开发了在保持原始表型的同时促进软骨细胞增殖的方法。本研究检验了以下假设：基于胶原的微载体是成骨细胞和软骨细胞繁殖的最合适制剂。

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