首页> 外文会议>Society for Biomaterials Transaction Annual Meeting vol.29 pt.2 >MAP Cell Binding Domain to Attach R28 Retinal Stem Cells to RCS Eyecups Over Time
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MAP Cell Binding Domain to Attach R28 Retinal Stem Cells to RCS Eyecups Over Time

机译:MAP细胞结合域将R28视网膜干细胞随时间附在RCS眼罩上

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Several previous structural and cellular attachment analyses were performed on mussel adhesive protein (MAP) and derivatives. MAP is marketed as a cellular attachment agent for a variety of cell types to various surfaces (1 and references therein). The MAP-repeating decamer (MAP-RD; A-K-P-S-Y-HYP-HYP-T-DOPA-K) and other MAP-derived peptides suggest that the L-DOPA residues are responsible for surface attachment ability. It has also been shown that the lysine-alanine-lysine (K-A-K) residues permit the cellular attachment to MAP films. Structural analysis of several peptides derived from MAP indicated that K-A-K amino acid segments were structurally conserved (2). Cyclic peptides were then designed that contained L-DOPA for the surface adhesion process and the K-A-K regions cellular attachment (DOPA-G-C-G-G-K-A-K-G-C [cycDOPA]). It was shown that cycDOPA formed thin uniform films capable of specific cellular attachment of MOLT-4 cells (2).
机译:对贻贝粘附蛋白(MAP)及其衍生物进行了几种先前的结构和细胞附着分析。 MAP作为细胞附着剂在市场上销售,用于各种类型的细胞到各种表面(1和其中的参考文献)。重复MAP的decamer(MAP-RD; A-K-P-S-Y-HYP-HYP-T-DOPA-K)和其他MAP衍生的肽表明L-DOPA残基负责表面附着能力。还已经表明,赖氨酸-丙氨酸-赖氨酸(K-A-K)残基允许细胞附着于MAP膜。几种衍生自MAP的肽的结构分析表明,K-A-K氨基酸区段在结构上是保守的(2)。然后设计包含L-DOPA用于表面粘附过程和K-A-K区细胞附着的环肽(DOPA-G-C-G-G-K-A-K-G-C [cycDOPA])。结果表明,cycDOPA形成了均匀薄的膜,能够对MOLT-4细胞进行特定的细胞附着(2)。

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