Molecular Detection of Proteins on Polymer Materials by Atomic Force Microscopy

机译：原子力显微镜对高分子材料中蛋白质的分子检测

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Molecular detection of individual proteins in multi-protein films on polymers is important for understanding blood material interactions. Adhesion mode AFM can be used to detect a specific protein type in a dual protein layer created by microcontact printing. Patterns were not as easily visualized on the polymers as they were previously on mica due to difficulties in μCP on polymers as well as large background forces.Mica was used to test the utility of gold conjugated anti-fibrinogen as an additional technique for protein detection. Phase images showed clear patterns following immunolabeling and can be obtained at higher resolution compared to the adhesion techniques.

机译：对聚合物上的多蛋白膜中的单个蛋白进行分子检测对于理解血液物质的相互作用非常重要。粘附模式AFM可用于检测通过微接触印刷产生的双重蛋白质层中的特定蛋白质类型。由于在聚合物上的μCP困难以及背景力大，因此在聚合物上观察到的图案不像以前在云母上那样容易观察到。云母用于测试金共轭抗纤维蛋白原作为蛋白质检测的另一种技术的效用。相图像显示免疫标记后清晰的模式，并且与粘附技术相比，可以更高的分辨率获得。

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《Society for Biomaterials Transaction Annual Meeting vol.29 pt.2》|2006年|614|共1页
会议地点 Pittsburgh,PA(US)
作者
Pranav Soman; Zachary Rice; Li-Chong Xu; Christopher A. Siedlecki;
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The Pennsylvania State University Hershey PA 17033;

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3. Atomic force microscopy detection of molecular complexes in multiprotein P450cam containing monooxygenase system [J] . Vadim Yu. Kuznetsov, Yuri D. Ivanov, Victor A. Bykov, Proteomics . 2002,第12期

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4. Molecular Detection of Proteins on Polymer Materials by Atomic Force Microscopy [C] . Pranav Soman, Zachary Rice, Li-Chong Xu, Society for Biomaterials Transaction of the 31st Annual Meeting vol.XXIX pt.2 . 2006

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5. Characterization of the molecular structure and mechanical properties of polymer surfaces and protein /polymer interfaces by sum frequency generation vibrational spectroscopy and atomic force microscopy [D] . Koffas, Telly Stelianos 2004

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6. Can non-mechanical proteins withstand force? Stretching barnase by atomic force microscopy and molecular dynamics simulation. [O] . R B Best, B Li, A Steward, 2001

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Molecular Detection of Proteins on Polymer Materials by Atomic Force Microscopy

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