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APPLICATION OF SOL-GEL METHOD FOR SYNTHESIS OF A BIOSENSITIVE POLYSILOXANE MATRIX

机译:溶胶-凝胶法在合成具有敏感性的聚硅氧烷基质中的应用

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Sol-gel method was used to synthesize polysiloxane hydrogel with encapsulated urease (immobilization degree in the range of 79-88%) which preserved the enzymatic activity at the level of 56-84%. The nature of functional groups was shown to influence the pore structure parameters. Immobilization degree and preservation of adsorbed urease activity depend on the structural-adsorption characteristics of matrices. The possibility of "double immobilization" of urease on silica gel by sol-gel method and the opportunity of reuse of the synthesized formulations was investigated. Urease immobilization on the surface of magnetite (FeO·Fe_2O_3) was also studied by adsorption method.
机译:采用溶胶-凝胶法合成了包封有脲酶(固定化度为79-88%)的聚硅氧烷水凝胶,其酶活保持在56-84%的水平。官能团的性质显示出会影响孔结构参数。固定化程度和吸附尿素酶活性的保留取决于基质的结构吸附特性。研究了通过溶胶-凝胶法将脲酶“双重固定”在硅胶上的可能性以及合成制剂可重复使用的机会。还通过吸附法研究了将脲酶固定在磁铁矿(FeO·Fe_2O_3)上的方法。

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