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首页> 外文会议>SPE Asia Pacific improved oil recovery conference (APIORC 01) >Microbial EOR Laboratory Studies on the Microorganisms Using Petroleum Hydrocarbon as a Sole Carbon Source
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Microbial EOR Laboratory Studies on the Microorganisms Using Petroleum Hydrocarbon as a Sole Carbon Source

机译：以石油烃为唯一碳源的微生物的微生物EOR实验室研究

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摘要

Eight kinds of facultative anaerobes using petroleum hydrocarbonrnas sole carbon source are screened out from hundredsrnof oil-bearing waste water samples based on physical and chemicalrncondition of Daqing oil field. Systematic biochemicalrnproperty evaluation and core-flooding experiment show thatrnthe eight kinds of facultative anaerobes can grow and reproducernon crude oil under anerobic condition. They also can degraderncrude oil and decrease its viscosity, produce activernsubstance and reduce the interfacial tension (IFT) betweenrnfermented solution and crude oil. Chromatographical analysisrnshows that the light component of oil which has treated withrnbacteria increases by 30%, ΣC21~-/Σ C22~+ increases by 54%.rnThe oil movability got better. Pr C17 and PhC18rnincreases by 55% and 57% respectively. The PH value of thernfermented solution reduces from 7 to 5. The acid content inrnthe fermented solution increases from 17.57mg/L torn948.83mg/L. The IFT between fermented solution and crudernoil reduces form 35.67mN/m to 8.10mN/m. The viscosity ofrncrude oil decreases from 28.1mPa?s to 18.06mPa?s. The oilrnrecovery by the microbial flooding is about 10% OOIP overrnthat of the water flooding. How the microbes displace thernresidual oil was directly observed by an electronicrnmicroscope during a microscopic core flooding experiment.
机译：根据大庆油田物化条件，从含数百个含油废水的样品中筛选出八种利用石油烃类唯一碳源的兼性厌氧菌。系统的生化性质评价和岩心驱油实验表明，在有氧条件下，八种兼性厌氧菌都可以生长和繁殖。它们还可以降解原油并降低其粘度，产生活性物质并降低发酵液和原油之间的界面张力（IFT）。色谱分析结果表明，经细菌处理的油的轻质成分增加了30％，ΣC21〜-/ΣC22〜+的增加了54％。 Pr / nC17和Ph / nC18rn分别增加了55％和57％。发酵液的PH值从7降低到5。发酵液中的酸含量从17.57mg / L增加到948.83mg / L。发酵溶液和粗丁腈之间的IFT从35.67mN / m降低到8.10mN / m。原油的粘度从28.1mPa？s降低到18.06mPa？s。微生物驱油的采收率比水驱油的采出率高出约10％。在微观岩心驱油实验中，电子显微镜直接观察了微生物如何驱除残留的油。

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