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首页> 外文会议>High-Speed Biomedical Imaging and Spectroscopy III: Toward Big Data Instrumentation and Management >Multi-channel imaging cytometry with a single detector
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Multi-channel imaging cytometry with a single detector

机译：单通道多通道成像细胞仪

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Multi-channel microscopy and multi-channel flow cytometry generate high bit data streams. Multiple channels (both spectral and spatial) are important in diagnosing diseased tissue and identifying individual cells. Omega Optical has developed techniques for mapping multiple channels into the time domain for detection by a single high gain, high bandwidth detector. This approach is based on pulsed laser excitation and a serial array of optical fibers coated with spectral reflectors such that up to 15 wavelength bins are sequentially detected by a single-element detector within 2.5 |is. Our multichannel microscopy system uses firmware running on dedicated DSP and FPGA chips to synchronize the laser, scanning mirrors, and sampling clock. The signals are digitized by an NI board into 14 bits at 60MHz - allowing for 232 by 174 pixel fields in up to 15 channels with 10x over sampling. Our multi-channel imaging cytometry design adds channels for forward scattering and back scattering to the fluorescence spectral channels. All channels are detected within the 2.5 μs - which is compatible with fast cytometry. Going forward, we plan to digitize at 16 bits with an A-to-D chip attached to a custom board. Processing these digital signals in custom firmware would allow an on-board graphics processing unit to display imaging flow cytometry data over configurable scanning line lengths. The scatter channels can be used to trigger data buffering when a cell is present in the beam. This approach enables a low cost mechanically robust imaging cytometer.
机译：多通道显微镜和多通道流式细胞仪产生高位数据流。多个通道（频谱通道和空间通道）在诊断患病组织和识别单个细胞中都很重要。欧米茄光学公司已经开发出了将多个通道映射到时域以通过单个高增益，高带宽检测器进行检测的技术。此方法基于脉冲激光激发和涂覆有光谱反射器的光纤的连续阵列，这样一来，单元素检测器可在2.5秒内依次检测多达15个波长段。我们的多通道显微镜系统使用在专用DSP和FPGA芯片上运行的固件来同步激光，扫描镜和采样时钟。 NI板将这些信号在60MHz处数字化为14位-允许在多达15个通道中以232乘174像素的像素场进行10倍过采样。我们的多通道成像细胞仪设计为荧光光谱通道增加了用于正向散射和反向散射的通道。在2.5μs内检测到所有通道-与快速细胞计数法兼容。展望未来，我们计划通过将A-to-D芯片连接到定制板上来以16位数字化。在定制固件中处理这些数字信号将允许车载图形处理单元在可配置的扫描线长度上显示成像流式细胞仪数据。当光束中存在单元时，散射通道可用于触发数据缓冲。这种方法使低成本的机械坚固的成像细胞仪成为可能。

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