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首页> 外文会议>Sugar Technologists' Association of India Annual Convention; 20030822-20030824; Orissa; IN >DECOLOURISATION OF DISTILLERY SPENTWASH BY PSEUDOMONAS PUTIDA
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DECOLOURISATION OF DISTILLERY SPENTWASH BY PSEUDOMONAS PUTIDA

机译：假单胞菌对牙膏洗消脱色

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摘要

Microbial decolourisation is seen as environmentally friendly technique for removing the colouring compounds from distillery waste water. Some groups of fungi possess decolourisation ability either via adsorption of colouring components by mycelia or via production of active oxygen, from hydrogen peroxide in a process coupled to sugar oxidase, which degrades melanoidtns and thus reduces the colour of distillery effluent. In the present study, feasibility of using Pseudomonas putida for decolourisation of distillery effluent has been demonstrated. This strain had the ability to extra-cellularly convert glucose into gluconic acid and hydrogen peroxide. The hydrogen peroxide, secondarily produced by this enzymatic oxidation decolourised the distillery effluent by oxidizing melanoidins. Pseudomonas putida immobilized in calcium alginate beads showed two and a half fold more decolourisation than free cells. The concentration of glucose, cell density and pH of the medium were found to be critical for decolourisation. The improved colour removal efficiency was obtained by replenishment of glucose periodically at six hours of intervals. Colour reducing ability of Pseudomonas putida was also obserued when lactose or whey was added to the distillery effluent. Whey is the waste from cheese manufacturing industry and contains lactose, which is catabolised by Pseudomonas putida into glucose and galactose. Glucose oxidase in Pseudomonas putida transforms glucose, produced after the hydrolysis of lactose, into gluconic acid and hydrogen peroxide which leads to reduction in colour of the distillery effluent.
机译：微生物脱色被视为从酿酒厂废水中去除着色化合物的环保技术。某些真菌通过菌丝体吸收着色成分或通过与糖氧化酶偶联的过程中过氧化氢产生活性氧而产生脱色能力，该过程会降解黑素，从而降低酒厂废水的颜色。在本研究中，已证明了使用恶臭假单胞菌对酒厂废水进行脱色的可行性。该菌株具有将细胞外葡萄糖转化为葡萄糖酸和过氧化氢的能力。通过该酶促氧化第二次产生的过氧化氢通过氧化黑素而使酒厂废水脱色。固定在藻酸钙珠粒中的恶臭假单胞菌显示的脱色比游离细胞多两倍半。发现培养基的葡萄糖浓度，细胞密度和pH对脱色至关重要。通过以六个小时为间隔定期补充葡萄糖，可以提高脱色效率。当将乳糖或乳清添加到酒厂废水中时，恶臭假单胞菌的减色能力也被消除。乳清是奶酪制造业的废物，其中含有乳糖，乳糖被恶臭假单胞菌分解为葡萄糖和半乳糖。恶臭假单胞菌中的葡萄糖氧化酶将乳糖水解后产生的葡萄糖转化为葡萄糖酸和过氧化氢，从而导致酒厂废水的颜色降低。

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