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首页> 外文会议>Symposium on Environmental Toxicology and Risk Assessment: Biomarkers and Risk Assessment-Fifth Volume 3-5 April 1995 in Denver, Colorado. >Alterations in chicken embryonic development as a sensitive indicator of 2, 3, 7, 8-tetrachlorodibenzo-p-dioxin exposure
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Alterations in chicken embryonic development as a sensitive indicator of 2, 3, 7, 8-tetrachlorodibenzo-p-dioxin exposure

机译：鸡胚胎发育变化是2、3、7、8-四氯二苯并二恶英暴露的敏感指标

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摘要

2, 3, 7, 8-Tectrachlorodibenzo-p-dioxin (2, 3, 7, 8-TCDD) exposure may be detected by chemical analysis or by its biological effects on in viov or in vitro systems. Chemical analysis is expensive and does not give indications of the bioavailability of the material to an oranism. Dosing an in vitro system with an extract obtained from an environmental sample has numerous advantages. This method is relatively cheap, quite sensitive, and can be used to generate quantitative dose response relationships as well as quantitative structure activity relationships. Olne method that is currently being used in our laboratiories and others is a whole embryo model which consists of fertile hen's eggs injected on day 0. The egges are incubated for either 48 or 96 hours and the embryos removed and examined for malformations. In addition, alterations in the vitelline vasculature are examined in the 96 hour embryos. This model is able to detect significant 2, 3, 7, 8-TCDD effects at levels of 2 - 20 pg/g egg (ppt), depending on the endpoint. The results of thins study were compared to results of the H4IIE assay based on EROD iinduction. Although the H4IIE assay is more sensitive, the egg injection method provides more data on effects.
机译：通过化学分析或其对活体或体外系统的生物学影响，可以检测到2、3、7、8-四氯二苯并-对-二恶英（2、3、7、8-TCDD）的暴露。化学分析是昂贵的，并且不能提供该材料对动物的生物利用度的指示。用从环境样品中获得的提取物对体外系统给药具有许多优点。该方法相对便宜，相当灵敏，并且可以用于生成定量的剂量反应关系以及定量的结构活性关系。目前在我们的实验室和其他实验室中使用的Olne方法是一个完整的胚胎模型，该模型由在第0天注入的可育母鸡卵组成。将卵孵育48或96小时，取出胚胎并检查其畸形。另外，在96小时的胚胎中检查卵黄脉管系统的改变。该模型能够根据端点在2-20 pg / g鸡蛋（ppt）的水平上检测到显着的2、3、7、8-TCDD效应。将Thins研究的结果与基于EROD诱导的H4IIE分析的结果进行了比较。尽管H4IIE分析更灵敏，但卵注射方法可提供更多有关作用的数据。

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