16 Culture of HUman Skin SHeet for Wound Therapy

机译：16人皮肤文化介绍伤口治疗

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Thailand has annually several thousand cases of burn including serious burn patients that is life-threatening. Among these, roughly 40 percent of the partients lost their lives due to lack of appropriate skin substitute or skin grafting. However, recent development in cell culture technique has enable one alternative solution to the problem of this serious shortage of skin grafts by mean of cultured skin system. This involves the primary culture of epidermal human keratinocytes for epidermal reconstruction and grafting of the cultured skin sheet to the wound site as replacement of the lost skin. The material used for cell culture was normal cell culture flasks and petri dishes, with special treatment for culture of anchorage-dependent cells. Silk protein has been considered suitable as coating material for skin cell culture but has not been tried out in this experiment.

机译：泰国每年有数千起烧伤病例，包括严重威胁生命的烧伤患者。其中，约40％的患者由于缺乏合适的皮肤替代品或植皮而丧命。然而，细胞培养技术的最新发展已经使得通过培养的皮肤系统能够解决皮肤移植物严重短缺的问题的一种替代解决方案。这涉及用于表皮重建的表皮人类角质形成细胞的原代培养以及将培养的皮肤片移植到伤口部位以替代失去的皮肤。细胞培养所用的材料是普通细胞培养瓶和培养皿，并经过特殊处理以培养锚定依赖性细胞。蚕丝蛋白被认为适合作为皮肤细胞培养的包衣材料，但尚未在该实验中进行尝试。

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《Takasaki Symposium on Radiation Processing of Natural Polymers November 23 and 24, 2000, JAERI, Takasaki, Japan》|2000年|p.116|共1页
会议地点 Takasaki(JP)
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Nares Damrongchai;
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正文语种 eng
中图分类农业基础科学;
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4. 16 Culture of HUman Skin SHeet for Wound Therapy [C] . Nares Damrongchai, Japan Atomic Energy Research Institute Takasaki symposium on radiation processing of natural polymers . 2001

机译：16伤口治疗人体皮层培养
5. Evaluation of human skin substitute for burn wound coverage based on cultured epidermal autograft. [D] . Lam, Ping Kuen. 1999

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7. Clinical identification of bacteria in human chronic wound infections: culturing vs. 16S ribosomal DNA sequencing [O] . 2012

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8. Preparation of Pure Epithelial Skin Cultures Intended for Coverage of Wound Surfaces. [R] . montes de oca, hector 1974

机译：用于覆盖伤口表面的纯上皮皮肤培养物的制备。

16 Culture of HUman Skin SHeet for Wound Therapy

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