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首页> 外文会议>The 2nd International Conference on Bioinformatics and Biomedical Engineering(iCBBE 2008)(第二届生物信息与生物医学工程国际会议）论文集 >Comparison of Defense Related Enzyme between Oligochitosan Induced Protein Kinase Gene Silenced Transgenic Tobacco and Wild Type Tobacco
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Comparison of Defense Related Enzyme between Oligochitosan Induced Protein Kinase Gene Silenced Transgenic Tobacco and Wild Type Tobacco

机译：低聚壳聚糖诱导的蛋白激酶基因沉默转基因烟草与野生型烟草防御相关酶的比较

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摘要

Oligochitosan (OC) could regulate plant defense responses in many aspects, but the basis signal transduction pathway is still unclear. In this study, we use transgenic (TG) tobacco as plant material whose OIPK gene was inhibited by antisense transformation, to identify the role oligochitosan induced protein kinase (OIPK) played in OC treated tobacco plants. Pathogen-related (PR) proteins enzymes activity assays and reverse transcriptional polymerase (RT-PCR) were conducted to compare the differences between TG and the corresponding wild type (WT) tobacco plants. Firstly, it was validated that OIPK could induce Phenylalanine ammonialyase( PAL, E.C. 4.3.1.5) and peroxidase (POD, E.C. 1.11.1.7 ) activities in respect to both activation time and activation fold after OC treatment, while OIPK had no influences on polyphenol oxidase (PPO, E.C.1.14.18.1) activity. Secondly, it was found that in TG tobacco, there was nearly just basis pal transcriptional expression after OC treatment, which showed that OIPK could determine pal gene expression in some sense. Thirdly, OIPK could enhance defense related gene transcription chi and glu activation intension and rapidity.
机译：寡聚壳聚糖（OC）可以在许多方面调节植物防御反应，但信号转导的基础途径尚不清楚。在这项研究中，我们使用转基因（TG）烟草作为其OIPK基因被反义转化抑制的植物材料，以确定寡聚壳聚糖诱导的蛋白激酶（OIPK）在OC处理的烟草植物中的作用。进行了病原相关（PR）蛋白质酶活性测定和逆转录聚合酶（RT-PCR），以比较TG和相应的野生型（WT）烟草植物之间的差异。首先，证实了OIPK在OC处理后可诱导苯丙氨酸解氨酶（PAL，EC 4.3.1.5）和过氧化物酶（POD，EC 1.11.1.7）的活性与活化时间和活化倍数有关，而OIPK对多酚没有影响氧化酶（PPO，EC1.14.18.1）活性。其次，发现在TG烟草中，OC处理后几乎只有基础pal转录表达，这表明OIPK可以在某种意义上决定pal基因的表达。第三，OIPK可以增强防御相关基因的转录和glu激活的强度和速度。

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