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Speckle-based phase retrieval applied to 3D microscopy

机译:基于斑点的相位检索应用于3D显微镜

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摘要

Nowadays Digital holographic microscopy (DHM) is used for three dimensional imaging of micro-objects. But DHM is a technique which requires the interference between the object beam and a known background, known as the reference beam. The two-beam nature of this makes it prone to external vibrations as well as a tedious adjustment of beam ratios to achieve high fringe contrast. Iterative phase retrieval techniques reconstruct the wavefront from the intensity sampled at several axial planes. This method is an attractive alternative to digital holographic methods, mainly because it is a single beam technique. The wavefront reconstruction is achieved by using the sampled intensity matrices in an appropriate diffraction integral. Angular spectrum propagation approach applied to scalar diffraction theory is used for propagating the wavefront between sampling planes. Here an overview of phase retrieval technique from multiple intensity sampling applied to 3D microscopy is provided.
机译:如今,数字全息显微镜(DHM)用于对微型对象进行三维成像。但是DHM是一种技术,需要在目标光束和已知背景(称为参考光束)之间进行干涉。这种双光束的特性使其易于受到外部振动的影响,并且繁琐地调整光束比以实现高条纹对比度。迭代相位检索技术从在几个轴向平面上采样的强度重建波前。该方法是数字全息方法的一种有吸引力的替代方法,主要是因为它是单光束技术。通过在适当的衍射积分中使用采样强度矩阵可以实现波前重建。将应用于标量衍射理论的角谱传播方法用于在采样平面之间传播波前。在此提供了从应用于3D显微镜的多强度采样获得的相位检索技术的概述。

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