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首页> 外文会议>Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing VI >Confocal laser microscopy of the in-vivo human skin revisited
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Confocal laser microscopy of the in-vivo human skin revisited

机译：激光共聚焦显微镜在体内人类皮肤

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Abstract: A new generation of confocal laser microscope, designed to image the human skin in vivo, improves the resolution, contrast and spectroscopic facilities as compared to the previous Tandem Scanning Microscope (TSM) prototype. The new device has been built with an Oz module (Noran) equipped with the skin contact device, assuming a perfect stability of skin images in the horizontal plane. The Z displacement of the objective lens, mounted directly on the Oz module, is assumed by a piezo motor with a course of 350 micrometer. Moreover, the Oz module has been suspended on articulated arms to reach any part of the human body. The power of the Argon/Krypton laser source has been limited to 2.mW to secure safety and provides three visible wavelength: 488, 568, and 647 nm. The facility of instantly checking wavelength during in depth exploration of the skin optimizes the resolution and contrast of images as compared with the white light used in the TSM. Consequently, better image quality of the epidermis is obtained in the blue region with unexpected details of corneocytes and keratinocytes. The papillary dermis comprising the vascular network is advantageously observed with the red light. The fluorescence channel detector gives additional information on the penetration of fluorescent probes through the skin barrier. Optical sections are digitized (512 $MUL 480 $MUL 8 bit) at video rate, providing easy and fast measurements of the thickness of epidermal layers. The Silicon Graphics workstation generates a transparent volume of living human skin in less than 5 minutes. This powerful and convivial new design for imaging the in vivo human skin opens up new promises in skin research and in vivo skin pharmacology. !11
机译：摘要：新一代共聚焦激光显微镜旨在在体内对人体皮肤成像，与以前的串联扫描显微镜（TSM）原型相比，它提高了分辨率，对比度和光谱学设施。该新设备采用配备有皮肤接触设备的Oz模块（Noran）制成，假定在水平面上的皮肤图像具有完美的稳定性。直接安装在Oz模块上的物镜的Z轴位移由行程为350微米的压电马达假定。此外，Oz模块已经悬挂在关节臂上，可以到达人体的任何部位。氩/ K激光源的功率已限制为2.mW，以确保安全并提供三个可见波长：488、568和647 nm。与TSM中使用的白光相比，在皮肤深度探索过程中即时检查波长的功能可优化图像的分辨率和对比度。因此，在蓝色区域获得了表皮更好的图像质量，并且角质细胞和角质形成细胞的细节出乎意料。用红光有利地观察到包含血管网络的乳头状真皮。荧光通道检测器提供了有关荧光探针穿过皮肤屏障的其他信息。光学部分以视频速率数字化（512 $ MUL 480 $ MUL 8位），可轻松快速地测量表皮层的厚度。 Silicon Graphics工作站可在不到5分钟的时间内生成透明的人类皮肤。这种功能强大且富有生命力的新型人体成像成像设计为皮肤研究和体内皮肤药理学开辟了新的希望。 ！11

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