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首页> 外文会议>Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XIII; Progress in Biomedical Optics and Imaging; vol.7 no.13 >Closing the spectral gap - the transition from fixed-parameter fluorescence to tunable devices in confocal microscopy
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Closing the spectral gap - the transition from fixed-parameter fluorescence to tunable devices in confocal microscopy

机译：缩小光谱间隙-共聚焦显微镜中从固定参数荧光到可调设备的过渡

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摘要

Modern microscopy in life sciences is ruled by development and exploration of new dyes and stains (probes for histochemical staining, quantum dots, fluorescent proteins etc.) on one side, and technological improvements and innovations for fluorescence microscopy - especially high resolution and optical sectioning microscopy — on the other side. Concerning the technical innovations, several ingenious inventions have been made available for confocal microscopy. First, the acousto optical tunable filter, which allows switching and dimming of laser lines. Second the spectral detector, employing mirror sliders in front of the detectors which allow continuous tuning of the spectral emission band detected by the sensor. Third, the most challenging task: a substitute to the classical beam splitter - the device which is restricting fluorescence microscopy most. This was solved by introduction of the acousto optical beam splitter. The very last device which is still lacking flexibility is the laser source, operating only at non-equidistant frequencies and requiring a set of quite different laser sources as gas lasers, solid state lasers or diode lasers. A new approach by supercontinuum light sources is presented and discussed, which significantly enhances flexibility and coverage of the excitation spectra of typical, rare and natural fluorochromes.
机译：一方面，生命科学的现代显微镜学受到新染料和染色剂（用于组织化学染色的探针，量子点，荧光蛋白等）的开发和探索以及荧光显微镜技术的改进和创新（尤其是高分辨率和光学切片显微镜）的支配- 另一方面。关于技术创新，共聚焦显微镜已经获得了几项独创的发明。首先是声光可调滤波器，它可以对激光线进行切换和调光。第二种是光谱探测器，在探测器前面采用镜面滑块，可以连续调节传感器探测到的光谱发射带。第三，最具挑战性的任务：经典分束器的替代品-最限制荧光显微镜检查的设备。这是通过引入声光分束器解决的。仍然缺乏灵活性的最后一个设备是激光源，它仅以非等频率运行，并且需要一组非常不同的激光源，例如气体激光器，固态激光器或二极管激光器。提出并讨论了一种超连续谱光源的新方法，该方法显着增强了典型，稀有和天然荧光染料的灵活性和对激发光谱的覆盖范围。

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