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首页> 外文会议>Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XIII; Progress in Biomedical Optics and Imaging; vol.7 no.13 >Biological cell (pollen grain) refractive index tomography with digital holographic microscopy
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Biological cell (pollen grain) refractive index tomography with digital holographic microscopy

机译：数字全息显微镜对生物细胞（花粉粒）折射率层析成像

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摘要

This paper present a novel approach to perform the tomography of biological specimen based on Digital Holographic Microscopy (DHM). A hologram results from the interference between a reference wave and an object wave reflected from or transmitted through a sample. In the hologram, both amplitude and phase of the field transmitted through the object are registered. In DHM, the object field is recovered when the hologram is processed by a digitally computed replica of the reference wave, allowing quantitative measurement of both phase and amplitude. Phase measurements provide high accuracy optical path length measurements across the specimen along the optical axis. To proceed to a tomographic reconstruction of the refractive index of the sample based on this quantitative phase measurement, such 2-dimentionnal data must be recorded for different sample orientations covering an angle of 180° to cover all the object spatial frequencies in the reciprocal space. The representation of the data in function of the angle is known as a sinogram. The 3-dimentionnal refractive index can then be reconstructed from the sinograms by a filtered backprqjection algorithm. In our system, the specimen is inserted in a glass micropipette to permit its rotation. To our knowledge, a quantitative tomography of the refractive index of a pollen cell with a resolution in the micron range is presented for the first time.
机译：本文提出了一种基于数字全息显微术（DHM）进行生物标本层析成像的新方法。全息图是由参考波和从样品反射或透过样品的物波之间的干涉产生的。在全息图中，记录了通过物体传输的场的振幅和相位。在DHM中，当全息图由参考波的数字计算副本处理后，物场得以恢复，从而可以定量测量相位和幅度。相位测量可沿光轴在整个样本上提供高精度的光程长度测量。为了基于此定量相位测量对样品的折射率进行断层成像重建，必须针对覆盖180°角度的不同样品方向记录此类二维数据，以覆盖互易空间中的所有物体空间频率。以角度为函数的数据表示形式称为正弦图。然后可以通过过滤的反向注入算法从正弦图中重建3维折射率。在我们的系统中，将标本插入玻璃微量移液器中以使其旋转。据我们所知，首次提出了一种具有微米级分辨率的花粉细胞折射率的定量层析成像技术。

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