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Influence of the surface hydrophobicity on Fluorescence Correlation Spectroscopy measurements

机译:表面疏水性对荧光相关光谱测量的影响

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Fluorescence correlation spectroscopy (FCS) is a powerful experimental technique used to analyze the diffusion at the single molecule level in solution. FCS is based on the temporal autocorrelation of fluorescent signal generated by dye molecules diffusing through a small confocal volume. These measurements are mostly carried out in a chambered coverglass, close to the glass substrate. In this report, we discuss how the chemical nature of the glass-water interface may interact with the free diffusion of molecules. Our results reveal a strong influence, up to a few μm from the interface, of the surface hydrophobicity degree. This influence is assessed through the relative weight of the two dimension diffusion process observed at the vicinity of the surface.
机译:荧光相关光谱法(FCS)是一项功能强大的实验技术,用于分析溶液中单分子水平的扩散。 FCS基于染料分子扩散穿过小的共聚焦体积而产生的荧光信号的时间自相关。这些测量主要在靠近玻璃基板的带腔室的盖玻片中进行。在这份报告中,我们讨论了玻璃水界面的化学性质如何与分子的自由扩散相互作用。我们的结果表明,对表面疏水度的影响最大(距离界面只有几微米)。通过在表面附近观察到的二维扩散过程的相对权重可以评估这种影响。

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