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首页> 外文会议>Ultrasensitive and Single-Molecule Detection Technologies II; Progress in Biomedical Optics and Imaging; vol.8 no.21; Proceedings of SPIE-The International Society for Optical Engineering; vol.6444 >Naphthalene diimides as tunable fluorophores suitable for single molecule applications
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Naphthalene diimides as tunable fluorophores suitable for single molecule applications

机译：萘二酰亚胺作为适用于单分子应用的可调荧光团

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摘要

The photophysics of two new substituted aminopropenyl naphthalene diimide (SANDI) dyes are reported. The molecules exhibit many of the photophysical properties required for fluorescence labeling applications including high photostability and high fluorescence quantum yields (＞ 0.5) in the visible region of the spectrum. Furthermore, the emission is sensitive to the number of substituents attached to the aromatic core, and to the surrounding environment. For example, in toluene as solvent, the mono-allyl SANDI has an emission maximum at 550 nm, whereas the di-allyl SANDI emits at 630 nm. The fluorescence decay times are in the range of ～8 - 12 ns and the Forster critical distance for fluorescence resonance energy transfer (FRET) between the mono- and di-allyl SANDI derivatives is 4.1 nm for a random donor-acceptor orientation. Single molecules of the di-allyl SANDI embedded in poly(methyl methacrylate) films show very low yields of photobleaching and very few fluorescence intermittencies or "blinks". These compounds are ideal candidates for applications at the single molecule level, for example, as FRET labels.
机译：报道了两种新的取代的氨基丙烯基萘二酰亚胺（SANDI）染料的光物理性质。这些分子表现出了荧光标记应用所需的许多光物理特性，包括在光谱的可见光区具有高光稳定性和高荧光量子产率（＞ 0.5）。此外，该发射对连接至芳族核以及周围环境的取代基的数量敏感。例如，在甲苯作为溶剂中，单烯丙基SANDI在550 nm处有最大发射，而二烯丙基SANDI在630 nm处有发射。荧光衰减时间在约8-12 ns的范围内，对于单供体-受体取向，单烯丙基和二烯丙基SANDI衍生物之间的荧光共振能量转移（FRET）的Forster临界距离为4.1 nm。嵌入聚（甲基丙烯酸甲酯）薄膜中的二烯丙基SANDI的单分子显示出非常低的光致漂白产率和极少的荧光间歇性或“闪烁”。这些化合物是单分子水平应用的理想候选物，例如作为FRET标记。

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