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A high-throughput label-free cell-based biosensor (CBB) system

机译：高通量无标签细胞生物传感器（CBB）系统

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摘要

Cell-based biosensors (CBBs) have important applications in biosecurity and rapid diagnostics. Current CBB technologies have challenges including cell immobilization on the sensors, high throughput fabrication and portability, and rapid detection of responses to environmental changes. We address these challenges by developing an integrated CBB platform that merges cell printing technology, a lensless charge-coupled device (CCD) imaging system, and custom-developed cell image processing software. Cell printing was used to immobilize cells within hydrogel droplets and pattern these droplets on a microfluidic chip. The CCD was used to detect the morphological response of the immobilized cells to external stimuli (e.g., environmental temperature change) using lensless shadow images. The morphological information can be also detected by sensing a small disturbance in cell alignment, i.e., minor alignment changes of smooth muscles cells on the biosensors. The automatic cell alignment quantification software was used to process the cell images (microscopic image was used as an example) and calculate the cell orientation in seconds. The same images were also manually processed as a control to validate and characterize the integrated platform functionality. The results showed software can measure the cell morphology (i.e., orientation) in an automated way without the need for labeling (e.g., florescent staining). Such an integrated CBB system will allow fabrication of CBBs at high throughput as well as rapidly monitor and measure morphological cellular responses.
机译：基于细胞的生物传感器（CBB）在生物安全和快速诊断中具有重要的应用。当前的CBB技术面临挑战，包括细胞固定在传感器上，高通量制造和便携性以及对环境变化响应的快速检测。我们通过开发整合了细胞打印技术，无透镜电荷耦合器件（CCD）成像系统和定制开发的细胞图像处理软件的集成CBB平台来应对这些挑战。细胞印刷用于将细胞固定在水凝胶小滴内，并在微流控芯片上将这些小滴图案化。 CCD用于使用无透镜阴影图像检测固定化细胞对外部刺激的形态反应（例如，环境温度变化）。还可以通过感测细胞排列中的小干扰，即生物传感器上平滑肌细胞的较小排列变化来检测形态学信息。使用自动细胞比对定量软件处理细胞图像（以显微图像为例），并以秒为单位计算细胞方向。还对相同的图像进行了手动处理，以作为对照，以验证和表征集成平台的功能。结果表明，软件可以自动方式测量细胞形态（即方向），而无需标记（例如荧光染色）。这种集成的CBB系统将允许以高通量制造CBB，并快速监控和测量形态细胞反应。

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  • 来源
  • 会议地点 Orlando FL(US)
  • 作者

    Feng Xu; rnSangJun Moon; rnEvan Hefher; rnTurker Beyazoglu; rnAhmet E. Emre; rnTariq Manzur; rnUtkan Demirci;

  • 作者单位

    Bio-Acoustic MEMS in Medicine (BAMM) Laboratory, HST-Center for Biomedical Engineering,Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston,Massachusetts, USA;

    rnBio-Acoustic MEMS in Medicine (BAMM) Laboratory, HST-Center for Biomedical Engineering,Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston,Massachusetts, USA;

    rnBio-Acoustic MEMS in Medicine (BAMM) Laboratory, HST-Center for Biomedical Engineering,Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston,Massachusetts, USA Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge,Massachusetts, USA;

    rnBio-Acoustic MEMS in Medicine (BAMM) Laboratory, HST-Center for Biomedical Engineering,Department of Medicine, Brig;

  • 会议组织
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 TP212;
  • 关键词

    cell-based biosensor; cell printing; high throughput; CCD;

    机译：基于细胞的生物传感器；单元格打印；高产量CCD;

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