A Rapid, Flow-through, DNA Extraction Module for Integration into Microfluidic Systems

机译：快速流通的DNA提取模块，可集成到微流体系统中

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摘要

A reusable flow-through micropillar chip module for efficient, pathogen nucleic acid extraction, purification, and concentration was evaluated on large volume samples. The module was tested on aqueous samples of Francisella tularensis genomic DNA. Only 30 min were required to process a 75-ml sample, resulting in a 1000-fold concentration effect. The data demonstrates the potential of microfluidic approaches for flow-through processing, detection and genetic identification of pathogenic agents at low concentrations in real-world samples.

机译：在大体积样品上评估了可重复使用的流通式微柱芯片模块，以进行有效的病原体核酸提取，纯化和浓缩。该模块在土拉弗朗西斯菌基因组DNA的水性样品上进行了测试。只需30分钟即可处理75毫升样品，从而产生1000倍的浓缩效果。数据证明了微流体方法在现实样品中低浓度的病原体流通处理，检测和遗传鉴定中的潜力。

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来源
《μTAS 2002 Symposium on Micro Total Analysis Systems 2002 Vol.2 Nov 3-7, 2002 Nara, Japan》|2002年|p.697-699|共3页
会议地点 Nara(JP);Nara(JP)
作者
Phillip Belgrader; Kevin Ness; Farzad Pourahmadi; Kevin Brounstein; M. Allen Northrup; Gary Long;
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作者单位

Microfluidic Systems Inc., 3918-B Valley Ave., Pleasanton, CA, 94566;

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会议组织
原文格式 PDF
正文语种 eng
中图分类 Q8;
关键词
sample preparation; DNA; PCR; microfluidics;

机译：样品制备; DNA; PCR;微流控;
入库时间 2022-08-26 14:10:04

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5. Integration of a rapid DNA quantitation technique and solid phase extraction on a microfluidic device. [D] . Shrinivasan, Sushil. 2006

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6. Rapid microfluidic solid-phase extraction system for hyper-methylated DNA enrichment and epigenetic analysis [O] . Arpita De, Wouter Sparreboom, Albert van den Berg, 2014

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A Rapid, Flow-through, DNA Extraction Module for Integration into Microfluidic Systems

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