TOWARDS SINGLE-CELL-CONTROLLED ELECTROPORATION IN A MICROFLUIDIC DEVICE

机译：迈向微流控设备中的单细胞控制电

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We are developing an integrated microfluidic device that combines electroporation with impedance spectroscopy flow cytometry. We here report on the kinetics of fluorophore intake by individually lysed HEK cells and on the transfection of CHO cells confirmed by functional protein expression.

机译：我们正在开发一种将电穿孔与阻抗谱流式细胞仪相结合的集成微流控设备。我们在这里报告了单个裂解的HEK细胞摄取荧光团的动力学以及功能蛋白表达所证实的CHO细胞的转染。

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来源
《μTAS 2002 Symposium on Micro Total Analysis Systems 2002 Vol.2 Nov 3-7, 2002 Nara, Japan》|2002年|p.796-798|共3页
会议地点 Nara(JP);Nara(JP)
作者
Urban Seger; Shady Gawad; Alexander Scheer; Philippe Renaud;
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作者单位

STI-LMIS, EPFL, CH-1015 Lausanne, Switzerland;

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会议组织
原文格式 PDF
正文语种 eng
中图分类 Q8;
关键词
electropermeabilisation; impedance spectroscopy; dielectrophoresis;

机译：电导;阻抗谱;介电泳;
入库时间 2022-08-26 14:10:05

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1. Thermal loading in flow-through electroporation microfluidic devices [J] . Blanca del Rosal, Chen Sun, Despina Nelie Loufakis Lab on a chip . 2013,第15期

机译：流通式电穿孔微流控设备中的热负荷
2. Electroporation of cells in microfluidic devices: a review [J] . Fox MB, Esveld DC, Valero A, Analytical and bioanalytical chemistry . 2006,第3期

机译：微流控设备中细胞的电穿孔：综述
3. Scalable Device for Automated Microbial Electroporation in a Digital Microfluidic Platform [J] . ACS Synthetic Biology . 2017,第9期

机译：用于数字微流体平台中的自动微生物电穿孔的可扩展装置
4. TOWARDS SINGLE-CELL-CONTROLLED ELECTROPORATION IN A MICROFLUIDIC DEVICE [C] . Urban Seger, Shady Gawad, Alexander Scheer, International conference on miniaturized chemical and biochemical analysis systems . 2002

机译：在微流体装置中朝着单细胞控制电穿孔
5. Polymeric microfluidic devices: Development of thermoset polyester microfluidic devices and use of poly(dimethylsiloxane) devices for droplet applications. [D] . Fiorini, Gina S. 2007

机译：聚合物微流体装置：开发热固性聚酯微流体装置，并将聚二甲基硅氧烷装置用于液滴应用。
6. Controlled electroporation of the plasma membrane in microfluidic devices for single cell analysis [O] . Duoaud Shah, Milan Steffen, Lothar Lilge 2012

机译：用于单细胞分析的微流控设备中质膜的受控电穿孔
7. Thermal loading in flow-through electroporation microfluidic devices [O] . Del Rosal, Blanca, Sun, Chen, Loufakis, Despina Nelie, 2013

机译：流通式电穿孔微流体装置中的热负荷

TOWARDS SINGLE-CELL-CONTROLLED ELECTROPORATION IN A MICROFLUIDIC DEVICE

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