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Characterization of Microalgae Growth-Promoting Bacteria (MGPB) isolated from Wastewater

机译:从废水中分离的微藻促生细菌(MGPB)的表征

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摘要

Microalgae have recently attracted attention as a good alternative feedstock for biofuel production owing to their high growth rate and high liquids accumulation capabilities Microalgae biomass production has been demonstrated by using wastewater. Enhancing microalgae growth is critical to achieving a highly efficient microalgae biofuel production. We focused on microalgae growth-promoting bacteria (MGPB). However little is known about MGPB in wastewater. Therefore, the objectives of this study were to isolate and characterize MGPB from wastewater. We used 3 microalgae (Euglena gracilis, Chlamydomonas reinhardtii, and Chlorella vulgaris) as model microalgae and cultured them using wastewater. Seventy-three bacterial strains were isolated from the 3 microalgae (26 strains from E. gracilis, 29 strains from C. reinhardtii, and 18 strains from C. vulgaris). Almost all of isolated strains belonged to alpha-proteobacteria. We evaluated the effects of isolated strains on the growth of each host-microalgae species in sterile liquid medium and real wastewater sample. Some isolated strains significantly increase and accelerate host microalgae growth. Some isolated strain increase growth rate of microalgae up to 2 times or more. The results show that MGPB can be present in wastewaters and that the use of MGPB in microalgae culture can improve the efficiency of microalgae biofuel production.
机译:由于微藻的高生长速率和高液体积累能力,微藻作为生物燃料生产的良好替代原料已引起人们的关注,该微藻的生物质生产已通过使用废水得到证明。促进微藻生长对于实现高效微藻生物燃料生产至关重要。我们专注于微藻促进细菌(MGPB)。然而,关于废水中的MGPB知之甚少。因此,本研究的目的是从废水中分离和表征MGPB。我们使用3种微藻(Euglena gracilis,莱茵衣藻和普通绿藻)作为模型微藻,并使用废水进行培养。从3个微藻中分离出73个细菌菌株(来自细叶肠杆菌的26个菌株,来自莱茵梭菌的29个菌株和来自寻常型梭菌的18个菌株)。几乎所有分离出的菌株都属于α-蛋白细菌。我们评估了分离菌株对无菌液体培养基和实际废水样品中每种宿主微藻物种生长的影响。一些分离的菌株显着增加并加速宿主微藻的生长。一些分离的菌株将微藻的生长速率提高至2倍或更多。结果表明,MGPB可以存在于废水中,并且在微藻培养中使用MGPB可以提高微藻生物燃料生产的效率。

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  • 会议地点 Sapporom(JP)
  • 作者单位

    Integrated Graduate School of Medicine, Engineering, and Agricultural Sciences, University of Yamanashi, Yamanashi, 400-8511;

    Graduate Faculty of Interdisciplinary Reseach, University of Yamanashi,Yamanashi, 400-8511;

    Graduate Faculty of Interdisciplinary Reseach, University of Yamanashi,Yamanashi, 400-8511;

    Graduate Faculty of Interdisciplinary Reseach, University of Yamanashi,Yamanashi, 400-8511;

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  • 正文语种 eng
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