声明
摘要
Abstract
CONTENTS
Chapter 1 Introduction
1．2 Natural variation，flowering time，and maturity trait in soybean
1．3 Photoperiodism phenomenon-remarkable findings in plant science
1．4 Regulatory networks and genes influencing flowering time in plants
1．5 Establishment of soybean growth season
1．6 The purpose and significance of this study
Chapter 2 GmCOL Genes in Maturity and Flowering Time
2．1 Photoperiod，and relevant genes that influencing maturity and flowering
2．2 CONSTANS,and CONSTANS-Like genes
2．3 Functional，and evolutionary role of COL family gene in soybean
2．4 Flowering time regulated by day length
2．5 Co-relation between maturity，flowering，and CONSTANS genes
2．6 Regulatory role of COL family genes in photoperiod responsive in flowering in different species
2．7 Identification，and selection of determinate growth habit MG cultivars
Chapter 3 Materials and Methods
3．1 Phenotype investigation of soybean in different maturity groups
3．1．1 Experimental materials
3．1．2 Methods
3．1．5 Phenotypic evaluation of stem growth habits
3．2 Genetic Study
3．2．4 Genomic DNA extraction
3．2．5 Data mining, and primer design
3．2．6 PCR amplification and purification
3．2．7 Sequencing,and sequence data analysis
Chapter 4 Results and Analysis
4．1 Analysis of soybean varieties in different growth stages
4．1．1 Investigation results of growth period traits
4．1．2 Association of flowering,and maturity dates of soybean shown divergence
4．1．3 Clustering analysis
4．1．4 Analysis of soybean maturity group varieties grouth period
4．1．5 Soybean varieties matured differently with different beginning bloom days
4．2 Bioinformatics analysis
4．2．1 Construction，and analysis of phylogram of COL genes in soybean
4．2．2 Analysis and association of soybean determinacy
4．3 Sequencing results：Analysis of COL genes in soybean varieties with different growth stages
4．3．1 Gene sequencing results and genotype analysis
4．3．2 Genotype distribution of GmCOL family genes in soybean cultivars
4．3．3 Genotype number and genotype frequency of GmCOL genes
4．3．4 GmCOL genes maturity groups,and genotype distribution
4．3．5 Statistical analysis of genotypes and phenotypes
4．3．6 Association analysis GmCOL genotypes,and pairwise comparison effect
4．3．7 Soybean COLgene family exhibited different linkage disequilibrium
4．3．8 Haplotype analysis of 21 soybean GmCOLfamily genes
4．3．9 Haplotypes，and natural variation of flowering time of GmCOL family genes
Chapter 5 Discussion
5．1 Selected soybean cultivars from different maturity groups show diversity in flowering time，and maturity
5．2 Natural variation，growth period group system and genotypes at the GmCOL loci of 128 accessions
5．3 Conservation，and divergence of TFL1 homologs in soybean，and other species
5．4 Polymorphism of GmCOL family genes-association to flowering time，and maturity
Chapter 6 Summary and Conclusion
References
Appendixes
Resume
Acknowledgements
