文摘
英文文摘
独创性声明及学位论文版权使用授权书
Chapter 1 Introduction
1. Introduction of self-assemble monolayers
1.1 Introduction
1.2 Fabrication and mechanism of forming SAMs on gold substrate
1.3 Applications of self-assembled monolayers
2. Importance of this research
2.1 Surface pKa of SAMs
2.2 Electrochemical behavior of epinephrine at different SAMs
2.3 Kinetic analysis of immunoreaction on modified electrode
3. Prospective
4. References
Chapter 2. Determination of the surface isoelectric point of self-assembled molecular films on gold by voltammetry titration
1. Introduction
2. Experimental
2.1. Reagents and materials
2.2. Apparatus
2.3. Preparation of SAMs electrodes
3. Results and discussion
3.1 Charge-transfer of Fe(CN)63-/4- at gold electrode modified by 1,3-benzenedithiol
3.2 Determination of surface pK of SAM-modified Au electrode with acidic or basic groups
3.3 Determination of surface isoelectric point at gold electrode modified by L-Cys and PCA
4. Conclusions
5. References
Chapter 3 .Electrochemical behavior of epinephrine at different self-assembled monolayer on gold electrode
1. Introduction
2. Experimental
2.1 Reagents
2.2 Apparatus
2.3 Preparation of SAMs
3. Research of EP at two-component self-assembled monolayer of meso-2, 3-dimercaptosuccinic acid and penicillamine
3.1 Characterization of mixed SAM in a Fe(CN)63-/Fe(CN)64-solution
3.2 Characterization for the DMSA and PCA mixed (2:1) SAM by electrochemical impedance spectroscopy
3.3 Electrochemical response of EP at the mixed SAM electrodes
3.4 Effects of pH on the peak current and peak potential of EP
3.5 Effect of scan rate on the peak current
3.6 Determination of the electrochemical parameters
3.7 Stability and standard deviation of the mixed SAM electrode
3.8 Calibration plots
3.9 Tolerance of foreign substances
3.10 Analytical application
4. Research of EP at DTDPA/Au electrode.
4.1 Characterization of the DTDPA/Au SAM in a Fe(CN) 63-/Fe(CN) 64- solution
4.2 Characterization for the DTDPA/Au SAM by AC impedance spectroscopy
4.3 Electrochemical response of the DTDPA/Au sAM on the oxidation of EP
4.4 Effect of pH value
4.5 Effect of the scan rate on the peak current
4.6 Stability of the DTDPA SAM electrode
4.7 Calibration plots
4.8 Tolerance of foreign substances
4.9 Analytical application
5. References
Chapter 4 Thermodynamic and kinetic analysis of the interaction between hepatitis B surface antibody and antigen on SAMs electrode
1. Introduction
2. Experimental
2.1 Materials.
2.2 Apparatus.
2.3 Preparation and characterization of colloidal gold.
2.4 Electrochemical measurement.
3. Research on a gold electrode modified with cysteamine and colloidal gold via DPV
3.1 Antibody immobilization
3.2 Cyclic voltammetry characteristics on the modified electrode surface.
3.3 Impedance characteristics of antibody-antigen binding.
3.4 Effect of the incubation time of HBsAb modified electrode on DPV response of the HBsAg solution.
3.5 Effect of the incubation temperature of HBsAb modified electrode on DPV response of the HBsAg solution.
3.6 Kinetic analysis for the affinity binding of HBsAg
3.7 Thermodynamic analysis for the affinity binding of1z HBsAg.
4. Research on a gold electrode modified with 1,6-hexanedithiol and colloidal gold via EIS
4.1 Antibody immobilization.
4.2 Characteristics of modified monolayer.
4.3 Theoretical impedance.
4.4 Effect of the incubation time of HBsAb modified electrode on the HBsAg solution.
4.5 Effect of the incubation temperature of HBsAb modified electrode.
4.6 Kinetic analysis for the affinity binding of HBsAg.
5. Contrastive analysis of the affinity binding of HBsAg.
6. Conclusions
7. References
Conclusions
致谢
Appendix: The papers published during the graduate
