声明
Abstract
摘要
Table of Contents
List of figures
List of tables
Chapter 1：Introduction
1．1．Electrochemistry at Liquid／Liquid interfaces
1．2．Structure of Interface Between Two Immiscible Electrolytes Solutions
1．3．PolariZation of ITIES
1．3．1．Ideally non-polarizable interface
1．3．2．Ideally polarizable ITIES
1．3．3．Potential window
1．4． Charge transfer process across ITIES
1．4．1．Ion transfer across ITIES
1．4．2．Electron transfer
1．5．Applications of liquidlliquid interface
1．5．1．Analytical application
1．5．2．Biological，physiological and pharmaceutical applications
Chapter 2：Study of simple ion transfer of Acetylcholine across water／1，2-Dichloroethane interface
2．1．Introduction
2．2．Objectives and significance of the study
2．3． Experimental methods for studying ion transfer across liquid／liquid interface
2．3．1．Voltammetry
2．4．Materials and methods
2．4．1．Chemicals
2．4．2．Pulling micropipettes
2．4．3．Silanization of microplpettes
2．4．4．Electrochemical measurements
2．4．5．Electrochemical cell
2．5．Results and discussions
2．5．1．Cyclic and square wave voltammogram of Acetylcholine transfer across water／DCE interface
2．5．2．Difierential pulse voltammetry
2．6．Summary
Chapter3：Control release of acetylcholine to stimulate catecholamine exocytosis from rat pheocromocytoma (PC 12) cells
3．1． Introduction
3．1．1．Introduction to catecholamine
3．1．2．The PC 12 cell as a model for studying exocytosis
3．2．Electrochemical methods used to study neurotransmitter release
3．2．1．Carbon fiber amperommetry
3．2．2．Chronoamperometry／Chronocoulometry
3．3．Materials and methods
3．3．1．Solutions
3．3．2．Cell culture
3．3．3．Carbon fiber microelectrode
3．3．4．Electrochemical detection
3．4．Results and discussion
3．5．Summary
Chapter 4：Ion selective detection based on facilitated transfer by propylene carbonate across water／1，2-Dichloroethane interface
4．1．Introduction
4．2．Experimental section
4．2．1．Chemicals
4．2．2．Instrument and electrodes
4．2．3 Electrochemical cell and measurement
4．3．Result and Discussion
4．3．1． Study by Cyclic Voltammogram
4．3．2．Investigation of the possibility of fabrication of ion sensors
4．4．Summary
Concluding remarks and future perspectives
References
Acknowledgments
