Preliminary Investigation on the Photobiomodulation Of Respiratory Burst of Porcine Neutrophil and β-Amyloid Peptide Induced PC12 Apoptosis

华南师范大学硕士学位论文答辩合格证明

Acronym and Glossary

PREFACE

摘要

1 INTRODUCTION

1.1The Research History of Biomodulation of Monochromatic Visible Light

1.1.1 Low Intensity Laser Therapy (LILT)

1.1.2 Light emitting diodes

1.1.3 Controversial and unestablished mechanisms

1.2 Molecular Mechanism of Respiratory Burst in Neutrophils

1.2.1 The Respiratory Burst of Polymorphonuclear Lukocytes

1.2.2 Assemble andActivation of NADPH Oxidase

1.2.3 Mechanism of Respiratory Burst

1.2.4 NBT test and HVB test about respiratory burst activity of PMNs

1.3 Controversial and unestablished mechanisms about apoptosis and Alzheimer's disease

1.3.1 What is Alzheimer's Disease?

1.3.2 Biomedical research

1.3.3 The PC12 cell line

2. The effects of Low Intensity Monochromatic Visible Light at different doses on Respiratory Burst in Porcine Neutrophils and their possible mechanism

2.1 INTRODUCTION

2.2 MATERIAL AND METHODS

2.2.1. Chemicals and instruments

2.2.2 Neutrophils isolation

2.2.3 Irradiation

2.2.4 Assay of Superoxide Production

2.2.5 Fluorescent assay of H2O2 generation

2.2.6 ImmunoblotAnalysis

2.3 RESULT

2.3.1 Effect of He-Ne laser irradiation at different doses on O2- production by porcine polymorphonucleated neutrophils (PMN)

2.3.2 Effect of light emitting diode (LED) irradiation at different doses on respiratory burst of porcine polymorphonucleated neutrophils (PMN)

2.3.3 Fluorescent assay of porcine polymorphonucleated neutrophils (PMN) H2O2 generation

2.3.4 Western blot analysis of phosphorylation of PTK, PKC and PLC in porcine PMN.

2.4 DISCUSS

2.5. CONCLUSION

3. LED irradiation decreased the Aβ induced apoptosis of differentiated PC12 cells in vitro

3.1 INTRODUCTION

3.2 MATERIALAND METHODS

3.2.1 Chemicals and instruments

3.2.2 Cell cultures

3.2.3 Aβ (25-35)

3.2. 4 LED irradiation

3.2.5 DNA fragmentation assay

3.2.6 Flow cytometry analysis

3.2. 7 Fluorimetric analysis of mitochondrial membrane potential (△ Ψ)

3.2.8Data analysis

3.3 RESULTS

3.3.1 Experiments on PC12 cells exposed to Aβ(25-35) or/and LED irradiation

3.3.2 Analysis of internucleosomal DNA fragmentation

3.3.3 Mitochondrial changes upon exposure to Ab or, and LED irradiation

3.4 DISCUSSION

3.5 CONCLUSION

Overview

Publications (published and in preparation)

致 谢

REFERENCE

华南师范大学学位论文原创性声明及使用授权声明