Effects of combined exposure to formaldehyde and PM2.5 on brain of Balb/c mice asthma model

声明

Abstract

摘要

Contents

1．Introduction

1．1 Asthma

1．1．1 Allergic asthma characterization

1．1．2 Asthma impact on brain function

1．2 Environmental pollutants

1．2．1 Outdoor air pollutants

1．2．3 Indoor air pollutants

1．3 PM2．5

1．3．2 PM2．5 impact on respiratory system and asthma

1．3．3 PM2．5 neurological effects

1．4 Formaldehyde

1．4．1 Formaldehyde background and exposure sources

1．4．2 Health effects of formaldehyde exposure

1．4．3 Formaldehyde neurotoxicity

1．5 Aims of the study

2．Materials and methods

2．1 Experimental animals

2．2 Preparation and components analysis of PM2．5

2．3 Exposure method

2．4 Brain coefficient and histological assay

2．5 Preparation of brain tissue homogenate

2．6 Detection of reactive oxygen species

2．7 GSH assay

2．8 Malondialdehyde measurement

2．9 Analysis of NF-κB and IL-1β

2．10 Analysis of IL-6

2．11 Statistical analysis

3．Results

3．2 Final weight and brain coefficient

3．3 Oxidative damage

3．4 Inflammation

3．5 Histopathological changes in the brain

4．Discussion

Conclusion

References

Acknowledgement