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胡桃楸、狗枣猕猴桃和木贼抗肿瘤活性成分研究

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目录

声明

致谢

I. Introduction

1. What is cancer?

2. Cancer treatment methods

3. Natural products for cancer prevention and treatment

4. Anti-tumor constituents from the leaves, stem barks, and unripe fruits of Juglans mandshurica Maxim

5. Anti-tumor constituents from the roots of Actinidia kolomikta

6. Anti-tumor constituents from the aerial parts of Equisetum hiemale

II. Materials and methods

1. General experimental procedures

2. Plant materials

3. Extraction and isolation

4. Specific Rotation assay

5. Circular dichroism (CD) assay

6. Acid hydrolysis assay

7. Assay for cytotoxicity

III. Results and discussion

1. Structure elucidation of compounds 1-24 from the CH2Cl2 fraction of the leaves of Juglans mandshurica

2 Structure elucidation of compounds 25-37 from the CHCl3 fraction of the stem barks of Juglans mandshurica

3 Structure elucidation of compounds 38-48 from the EtOAc fraction of the stem barks of Juglans mandshurica

4 Structure elucidation of compounds 49-63 from the CHCl3 fraction of the unripe fruits of Juglans mandshurica

5 Structure elucidation of compounds 64-70 from the CHCl3 fraction of the roots of Actinidia kolomikta

6 Structure elucidation of compounds 71-82 from the EtOAc fraction of the aerial parts of Equisetum hiemale

7 Evaluation of Antitumor Activities

IV. Conclusion

参考文献

VI. Appendix

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摘要

本论文对胡桃楸(Juglans mandshurica Maxim.)、狗枣猕猴桃(Actinidia kolomikta Rupr. et Maxim.)和木贼(Equisetum hiemale L.)的抗癌活性成分进行了研究。
  1.发现胡桃楸叶的二氯甲烷提取物、茎皮的三氯甲烷及乙酸乙酯提取物、青果皮三氯甲烷提取物对人宫颈癌细胞(HeLa)、人肺癌细胞(A549)和人肝癌细胞 SK-Hep1显示了较强的细胞毒性。根据活性追踪法对胡桃楸叶二氯甲烷提取物、茎皮三氯甲烷及乙酸乙酯提取物、青果皮三氯甲烷提取物进行了提取、分离和纯化,共分离得到63个单体化合物,其中包括6个新化合物(1个酚苷类、2个共轭酮脂肪酸类和3个二芳基庚烷类化合物)。其余57个已知化合物分别为juglanin C(1),(+)-isosclerone(2),myricatomentogenin(3),(3R,7R)-3,7-dihydroxy-1-(4-hydroxyphenyl)-7-(4-hydroxy-3-methoxyphe nyl)heptane(5),4-hydroxy-1-tetralone(6),ferulic acid(7),caffeic acid methyl ester(8),1,3,5-trimethoxybenzene(9),(3R)-3-hydroxy-1-(4-hydroxyphenyl)-7-(4-hydroxy-3-methoxyphenyl)heptane(10),3,11(S),17-trihydroxy-2-methoxy-1,16-oxo-7,13-diphenyl-11-heptanol(11),4-hydroxybenzoic acid methyl ester(12),methyl p-hydroxycinnamate(13),β-Sitosterol(14),(6R,7E,9S)-9-Hydroxy-4,7-megastigmadien-3-one(15),sarmentof F(16),benzoic acid(17),rhein ethyl ester(18),5-hydroxy-1,3-dimethoxy-9,10-anthracenedione(19),vomifoliol(20),4,5-dihydroblumenol A(21),ferutlic acid(22),3(R)-hydroxy-1,7-bis(4-hydroxy-3-methoxypheyl)heptane(23),(+)-syringaresinol(24),(–)-regiolone(25),2-oxatrycyclo[13.2.2.13,7] eicosa-3,5,7(20),15,17,18-hexaen-10-16-diol(26),syringic acid(27),(11R)-3,11,17-trihydroxy-2-methoxy-1,16-oxo-7,13-diphenyl-11-heptanol(28),(S)-(8E,10E)-12-hydroxy-7-oxo-8,10-octadecadienoic acid methyl ester(29),stearic acid(30),juglanin A(31),myricatomentogenin(32),5-hydroxy-1,3-dimethoxy-9,10-anthracenedione(33),engelheptanoxide A(34),stigmast-4-en-3-one(35),(6R,7E,9S)-9-hydroxy-4,7-megastigmadien-3-one(36),rhein ethyl ester(37),5-hydroxy-2-methoxy-1,4-naphthoquinone(38),4,8-dihydroxy-1-tetralone(39),gallic acid methyl ester(40),6-O-galloyl-D-glucopyranose(43),1-O-galloyl-β-D-glucopyranose(44),1,2,6-trigalloylglucopyranose(45),1,2,3,6-tertagalloylglucopyranose(46),4'α,5',8'-trihydroxy-α-tetralone-5'-O-β-D-[6-O-(4

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