声明
TABLE OF CONTENTS
LIST OF FIGURES
LIST OF TABLES
ABSTRACT
摘要
ABBREVIATIONS
CHAPTER 1 LITERATURE REVIEW
Background
1．1 Taxonomy and nomenclature of Mycoplasma hyopneumoniae
1．2 Etiology and Growth characteristics of Mhyo
1．3 Transmission of Mycoplasma hyopneumoniae
1．4 Airborne transmission
1．5 Ecology，Biology and Pathogenesis of Mycoplasma hyopneumoniae
1．6 Economic impact of Mycoplasma hyopneumoniae in pig production
1．7 Methods of diagnosing Mycoplasma hyopneumoniae infection
1．8 Management of M．hyopneumoniae
Chapter 2 Comparative analysis of the detection and monitoring of Mycoplasma hyopneumoniae infection by Nested PCR(n-PCR)and Real time PCR(q-PCR)from field swine herds
Introduction
1 Materials and Methods
1．1 Sampling
1．2 DNA extraction
1．3 Detection of M．hyopneumoniae DNA
1．4 Results
1．5 Discussion
Chapter 3 Real Time PCR，Serology and Culture in the detection of Mycoplasma hyopneumoniae from field swine herds
1 Introduction
2 Materials and Methods
2．1 Materials
2．2 Sample collection and preparation
2．3 Sample analysis
3 Culture
4 Results
5 Discussion
Chapter 4 Detection of Mycoplasma hyopneumoniae antibodies in serum and oral fluids in pigs using ELISA
1 Introduction
2 Materials and Methods
2．1 Vaccination
3 Methodology
4 Results
5 Discussion
OVERALL CONCLUSIONS
INNOVATION
Publications
REFERENCES
ACKNOWLEDGEMENTS
